Dependence of swarming in Escherichia coli K-12 on spermidine and the spermidine importer

Dependence of swarming in Escherichia coli K-12 on spermidine and the spermidine importer
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DOI:
10.1111/j.1574-6968.2009.01552.x
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发表时间:
2009-05-01
影响因子:
2.1
通讯作者:
Benno, Yoshimi
Benno, Yoshimi
中科院分区:
生物学4区
文献类型:
--
作者:
Kurihara, Shin;Suzuki, Hideyuki;Benno, Yoshimi

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在先前的研究中,我们观察到多胺缺乏的奇异变形杆菌(speB::sm)在Luria-Bertani (LB)聚集板(LBSw) (LB, 0.5%葡萄糖,0.5%琼脂)上的聚集受到严重抑制,并阐明了胞外腐胺作为诱导奇异变形杆菌聚集的重要信号分子。然而,在LBSw平板上,大肠杆菌多胺缺乏菌株(δ - speab δ - spec)和亲本菌株一样大量聚集。我们报告了多胺缺乏大肠杆菌菌株的群体表型依赖于亚精胺和PotABCD,一个亚精胺进口商。
In a previous work, it was observed that the swarming of polyamine-deficient Proteus mirabilis (speB::sm) was severely inhibited on Luria-Bertani (LB) swarming plates (LBSw) (LB, 0.5% glucose, 0.5% agar), and it was clarified that extracellular putrescine was important as a signaling molecule for the induction of swarming in P. mirabilis. However, a polyamine-deficient strain (delta-speAB delta-speC) of Escherichia coli swarmed as well as the parental strain on LBSw plates. We report that the swarming phenotype of a polyamine-deficient E. coli strain is dependent on spermidine and PotABCD, a spermidine importer.