Transcriptomic analysis of Arabidopsis thaliana plants treated with the Ky-9 and Ky-72 histone deacetylase inhibitors
Transcriptomic analysis of Arabidopsis thaliana plants treated with the Ky-9 and Ky-72 histone deacetylase inhibitors
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DOI：
10.1080/15592324.2018.1448333
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发表时间：
2018-01-01
影响因子：
2.9
通讯作者：
Seki, Motoaki
中科院分区：
文献类型：
--
作者：
Huong Mai Nguyen;Sako, Kaori;Seki, Motoaki
组蛋白乙酰化在植物生长发育中起关键作用，受组蛋白乙酰转移酶（Histone acetyltransferase， HAT）和组蛋白去乙酰化酶（Histone deacetylase， HDAC）的拮抗作用调控。我们之前揭示了一些HDAC抑制剂赋予植物高盐胁迫耐受性。在本研究中，我们鉴定了两种HDAC抑制剂key -9和key -72，它们增强了拟南芥的高盐胁迫耐受性。key -9和key -72是结构相似的衣原菌蛋白类似物。但是，key -9对哺乳动物HDAC的体外抑制活性大于key -72。western blot结果显示，key -9和key -72增加了组蛋白H3的乙酰化水平，表明它们在植物中具有抑制HDAC的活性。我们进行了转录组学分析，以研究key -9和key -72如何增强高盐胁迫耐受性。虽然与key -72相比，key -9上调了更多基因的表达，但两种HDAC抑制剂诱导的基因表达模式相似。此外，key -9和key -72增加了花青素相关基因和小肽编码基因等耐高盐胁迫相关基因的表达。这些数据表明，HDAC抑制剂之间化学侧链的细微结构差异可以改变对HDAC蛋白的抑制作用，从而影响基因表达，从而不同程度地增强高盐胁迫的耐受性。
Histone acetylation plays a pivotal role in plant growth and development, and is regulated by the antagonistic relationship between histone acetyltransferase (HAT) and histone deacetylase (HDAC). We previously revealed that some HDAC inhibitors confer high-salinity stress tolerance in plants. In this study, we identified two HDAC inhibitors, namely Ky-9 and Ky-72, which enhanced the high-salinity stress tolerance of Arabidopsis thaliana. Ky-9 and Ky-72 are structurally similar chlamydocin analogs. However, the in vitro inhibitory activity of Ky-9 against mammalian HDAC is greater than that of Ky-72. A western blot indicated that Ky-9 and Ky-72 increased the acetylation levels of histone H3, suggesting they exhibit HDAC inhibitory activities in plants. We conducted a transcriptomic analysis to investigate how Ky-9 and Ky-72 enhance high-salinity stress tolerance. Although Ky-9 upregulated the expression of more genes than Ky-72, similar gene expression patterns were induced by both HDAC inhibitors. Additionally, the expression of high-salinity stress tolerance-related genes, such as anthocyanin-related genes and a small peptide-encoding gene, increased by Ky-9 and Ky-72. These data suggest that slight structural differences in chemical side chain between HDAC inhibitors can alter inhibitory effect on HDAC protein leading to influence gene expression, thereby enhancing high-salinity stress tolerance in different extent.