H-NS mediated compaction of DNA visualised by atomic force microscopy
H-NS mediated compaction of DNA visualised by atomic force microscopy
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DOI：
10.1093/nar/28.18.3504
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发表时间：
2000-09-15
影响因子：
14.9
通讯作者：
Goosen, N
中科院分区：
文献类型：
--
作者：
Dame, RT;Wyman, C;Goosen, N
大肠杆菌H-NS蛋白是一种参与基因调控和DNA压缩的核相关蛋白。为了更深入地了解DNA压缩的机理，我们应用原子力显微镜（AFM）研究了H-NS-DNA复合物的结构。在环状DNA分子上观察到两种不同水平的H-NS诱导的缩合。H-MS：诱导质粒大区域的侧向缩合。此外，还鉴定出掺入了大量DNA的大球状结构。这些球状结构的形成似乎不依赖于任何特定的序列。在AFM图像的基础上，提出了H-NS对染色体DNA的整体凝聚模型。
The Escherichia coli H-NS protein is a nucleoid-associated protein involved in gene regulation and DNA compaction. To get more insight into the mechanism:of DNA compaction we applied atomic force microscopy (AFM) to study the structure of H-NS-DNA complexes. On circular DNA molecules two different levels of H-NS induced condensation were observed. H-MS:induced lateral condensation of large regions of the:plasmid. In addition, large globular structures were identified that incorporated a considerable amount of DNA. The formation of these globular structures appeared not to be dependent on any Specific sequence, On the basis of the AFM images, a model for global condensation of the chromosomal DNA by H-NS is proposed.