Near-infrared spectroscopic measurement of physiological glucose levels in variable matrices of protein and triglycerides
Near-infrared spectroscopic measurement of physiological glucose levels in variable matrices of protein and triglycerides
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DOI：
10.1021/ac950751x
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发表时间：
1996-04-01
影响因子：
7.4
通讯作者：
Small, GW
中科院分区：
文献类型：
--
作者：
Pan, ST;Chung, H;Small, GW
利用5000 ~ 4000 cm（-1）近红外光谱的偏最小二乘回归分析，生成了1 ~ 20 nM浓度范围内葡萄糖的选择性校准模型。两个光谱数据集用于模拟甘油三酯和蛋白质在临床样品的变化。在一个数据集中使用三乙酸酯来模拟甘油三酯水平的变化，在第二个数据集中使用牛血清白蛋白（BSA）来模拟血液蛋白水平的变化。尽管这些基质成分具有很强的吸收带，重叠并掩盖了葡萄糖的吸收带，但可以生成成功的校准模型，没有证据表明基质成分的不同水平会导致预测偏差。此外，使用数字傅立叶滤波作为预处理步骤的好处是评估校准性能方面。所得到的校准模型在三乙酸乙酯和牛血清白蛋白基质中提供的预测标准误差分别为0.5和0.2 mM。从与校准数据集中不存在的蛋白质浓度相对应的光谱中证明了准确的葡萄糖预测。最后，由于三种物质的吸收带宽度相似，仅使用数字傅立叶滤波从牛血清白蛋白和三乙酸蛋白中分离葡萄糖信号的能力有限。
Selective calibration models are generated for glucose over the 1-20 nM concentration range by use of partial least-squares regression analysis of near-infrared spectra from 5000 to 4000 cm(-1). Two spectral data sets are used to simulate triglyceride and protein variations in clinical samples. Triacetin is used in one data set to simulate variations in triglyceride levels, and bovine serum albumin (BSA) is used in the second data set to simulate variations in blood protein levels, Although these matrix components possess strong absorption bands that overlap and overshadow the absorption bands of glucose, successful calibration models can be generated with no evidence of prediction bias caused by the different levels of the matrix components. Furthermore, the benefits of using digital Fourier filtering as a preprocessing step are evaluated in terms of calibration performance. The resulting calibration models provide standard errors of prediction of 0.5 and 0.2 mM in triacetin and BSA matrices, respectively. Accurate glucose predictions are demonstrated from spectra that correspond to protein concentrations not present in the calibration data set, Lastly, digital Fourier filtering alone is shown to have only limited ability to isolate glucose signals from those of BSA and triacetin due to similarities in the widths of the absorption bands of the three species.