Engineering and repair of diaphragm using biosheet (a collagenous connective tissue membrane) in rabbits
Engineering and repair of diaphragm using biosheet (a collagenous connective tissue membrane) in rabbits
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DOI：
10.1016/j.jpedsurg.2017.11.035
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发表时间：
2018-02-01
影响因子：
2.4
通讯作者：
Nakayama, Yasuhide
中科院分区：
文献类型：
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作者：
Suzuki, Keisuke;Komura, Makoto;Nakayama, Yasuhide
背景：假体补片可用于修复大面积先天性膈疝缺损，但可能与感染、复发和胸部畸形有关。生物膜（胶原结缔组织膜）已被用于再生医学。我们在兔模型上评估了Biosheets的功效。方法：将硅胶板包埋于家兔背部皮下袋内4周，制备生物片。在第1组（n=11）中，将Gore-Tex (R)片（1.8 x 1.8 cm）植入膈缺损。第二组（n=11）采用相同方法植入生物可吸收人工硬脑膜替代物Seamdura (R)。在第三组（n=14），生物片被自体移植到膈缺损。所有家兔在移植后3个月被安乐死以评估其移植状态。结果：1组肝突出5只（45%），2组8只（73%），3组3只（21%）。第2组与第3组间差异有统计学意义（P=0.017）。生物膜具有与天然隔膜相当的破裂强度和弹性模量。3组移植生物片的肌肉组织再生得到组织学证实。结论：生物膜可用于膈肌组织修复和置换。(c) 2017 Elsevier Inc.版权所有。
Background: Prosthetic patches can be used to repair large congenital diaphragmatic hernia defects but may be associated with infection, recurrence, and thoracic deformity. Biosheets (collagenous connective tissue membranes) have been used in regenerative medicine. We evaluated the efficacy of Biosheets in a rabbit model.Methods: Biosheets were prepared by embedding silicone plates in dorsal subcutaneous pouches of rabbits for 4 weeks. In group 1 (n=11), Gore-Tex (R) sheets (1.8 x 1.8 cm) were implanted into a diaphragmatic defect. In group 2 (n=11), Seamdura (R), a bioabsorbable artificial dural substitute, was implanted in the same manner. In group 3 (n=14), biosheets were autologously transplanted into the diaphragmatic defects. All rabbits were euthanized 3 months after transplantation to evaluate their graft status.Results: Herniation of liver was observed in 5 rabbits (45%) in group 1, 8 (73%) in group 2, and 3 (21%) in group 3. A significant difference was noted between groups 2 and 3 (P=0.017). Biosheets had equivalent burst strength and modulus of elasticity as native diaphragm. Muscular tissue regeneration in transplanted biosheets in group 3 was confirmed histologically.Conclusion: Biosheets may be applied to diaphragmatic repair and replacement of diaphragmatic muscular tissue. (c) 2017 Elsevier Inc. All rights reserved.