Failure of glucose and branched-chain amino acids to normalize brain glucose use in portacaval shunted rats.
Failure of glucose and branched-chain amino acids to normalize brain glucose use in portacaval shunted rats.
复制标题
葡萄糖和支链氨基酸未能使门静脉分流大鼠的脑葡萄糖使用正常化。
DOI：
10.1111/j.1471-4159.1986.tb00776.x
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发表时间：
1986
影响因子：
4.7
通讯作者：
Hawkins,RA
中科院分区：
文献类型：
--
作者：
Mans,AM;Davis,DW;Biebuyck,JF;Hawkins,RA
静脉输注葡萄糖或含支链氨基酸的葡萄糖4天后，由门腔静脉分流引起的大鼠脑和血浆氨基酸浓度的几个异常恢复到正常水平。为了评估这种治疗对大脑能量代谢的影响，在门腔静脉分流术后5周，使用[14C]葡萄糖和放射自显影测量局部脑葡萄糖使用量。在一个实验中，静脉注射葡萄糖或含有支链氨基酸的葡萄糖，为期4天。在另一项单独的实验中，口服治疗2周，除了葡萄糖的使用外，还测量了大脑中的单胺和氨基酸。没有提供其他食物；老鼠可以自由获得水。正常饲养的分流大鼠和假手术大鼠作为对照。两种类型的口服治疗都降低了血浆和大脑中高浓度的酪氨酸、苯丙氨酸和谷氨酰胺。不含氨基酸的葡萄糖使大脑色氨酸正常化。分流术后脑组织去甲肾上腺素、5-羟色胺(5-羟色胺)和5-羟吲哚乙酸水平明显升高。治疗对去甲肾上腺素没有影响，但葡萄糖饮食使吲哚恢复到正常范围。相比之下，静脉或口服治疗都不会影响大脑葡萄糖的使用，在所有被检查的大脑区域中，大脑葡萄糖的使用仍然保持着25%-30%的低水平。
Several abnormalities in brain and plasma amino acid concentrations caused by portacaval shunting in rats return toward normal after 4 days of intravenous infusion with either glucose or glucose with branched‐chain amino acids. To assess the effect of such treatment on brain energy metabolism, regional brain glucose use was measured using [14C]glucose and autoradiography, 5 weeks after portacaval shunting. In one experiment intravenous glucose or glucose with branched‐chain amino acids was given for 4 days. In a separate experiment the treatment was given orally for 2 weeks, and in addition to glucose use, brain monoamines and amino acids were measured. No other food was provided; the rats had free access to water. Normally fed shunted rats and sham‐operated rats served as controls. Both types of oral treatment lowered the high concentrations of tyrosine, phenylalanine, and glutamine in plasma and brain. Glucose without amino acids normalized brain tryptophan. Levels of brain norepinephrine, 5‐hydroxytryptamine (serotonin), and 5‐hydroxyindoleacetic acid were significantly raised after shunting. Treatment had no effect on norepinephrine but the glucose diet brought the indoles into the normal range. In contrast, neither intravenous nor oral treatment affected brain glucose use, which remained depressed by 25–30% in all brain areas examined.