Accumulation of DNA damage alters microRNA gene transcription in Arabidopsis thaliana.
Accumulation of DNA damage alters microRNA gene transcription in Arabidopsis thaliana.
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DNA损伤的积累改变了拟南芥中的microRNA基因转录
DOI：
10.1186/s12870-022-03951-9
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发表时间：
2022-12-12
影响因子：
5.3
通讯作者：
Zhang, Xiaoming
中科院分区：
文献类型：
--
作者：
Du, Juan;Liu, Yang;Lu, Lu;Shi, Jianfei;Xu, Longqian;Li, Qi;Cheng, Xiaofei;Chen, Jinfeng;Zhang, Xiaoming
microRNA（miRNAs）和其他表观遗传修饰在所有真核生物过程中起着重要作用。DNA损伤修复是维持不同生物体基因组完整性的关键过程。然而，miRNAs在DNA损伤修复过程中的反应尚不清楚。在本研究中，我们发现锌指DNA 3′-磷酸酯酶（ZDP）和AP内切核酸酶2（APE 2）这两个在主动DNA去甲基化和碱基切除修复（BER）中起重叠作用的基因的同时突变导致了全基因组范围的miRNAs改变。在zdp/ape 2中，新转录的miRNAs编码基因（MIRs）的转录本显著减少，表明ZDP和APE 2的突变在转录水平上影响了miRNAs的积累。此外，用DNA烷基化试剂甲磺酸甲酯（MMS）引入碱基损伤加速了zdp/ape 2中miRNA的减少。进一步突变双功能DNA糖基化酶/裂解酶甲酰胺嘧啶DNA糖基化酶（FPG），挽救了zdp/ape 2中miRNA的积累，表明DNA损伤修复中间体的积累诱导了miRNA的转录抑制。我们的研究表明，DNA损伤修复中间产物的积累通过抑制MIR转录来抑制miRNA的积累。在线版本包含补充材料，可通过10.1186/s12870-022-03951-9获得。
MicroRNAs (miRNAs) and other epigenetic modifications play fundamental roles in all eukaryotic biological processes. DNA damage repair is a key process for maintaining the genomic integrity of different organisms exposed to diverse stresses. However, the reaction of miRNAs in the DNA damage repair process is unclear. In this study, we found that the simultaneous mutation of zinc finger DNA 3′-phosphoesterase (ZDP) and AP endonuclease 2 (APE2), two genes that play overlapping roles in active DNA demethylation and base excision repair (BER), led to genome-wide alteration of miRNAs. The transcripts of newly transcribed miRNA-encoding genes (MIRs) decreased significantly in zdp/ape2, indicating that the mutation of ZDP and APE2 affected the accumulation of miRNAs at the transcriptional level. In addition, the introduction of base damage with the DNA-alkylating reagent methyl methanesulfonate (MMS) accelerated the reduction of miRNAs in zdp/ape2. Further mutation of FORMAMIDOPYRIMIDINE DNA GLYCOSYLASE (FPG), a bifunctional DNA glycosylase/lyase, rescued the accumulation of miRNAs in zdp/ape2, suggesting that the accumulation of DNA damage repair intermediates induced the transcriptional repression of miRNAs. Our investigation indicates that the accumulation of DNA damage repair intermediates inhibit miRNAs accumulation by inhibiting MIR transcriptions. The online version contains supplementary material available at 10.1186/s12870-022-03951-9.
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