Ultrasensitive detection of DNA hybridization using carbon nanotube field-effect transistors
Ultrasensitive detection of DNA hybridization using carbon nanotube field-effect transistors
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DOI：
10.1143/jjap.43.l1558
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发表时间：
2004-12-01
期刊：
影响因子：
--
通讯作者：
Tamiya, E
中科院分区：
文献类型：
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作者：
Maehashi, K;Matsumoto, K;Tamiya, E
我们已经使用碳纳米管场效应晶体管(CNTFET)实时灵敏地检测到DNA杂交。5‘端的氨基修饰的肽核酸(PNA)寡核苷酸以共价方式固定在Au的后门表面。对于11聚体PNA寡核苷酸探针，可以有效地检测到浓度低至6.8fM的全互补DNA。我们基于CNTFET的生物芯片是开发用于医疗、法医和环境诊断的集成、高通量、多路复用DNA生物传感器的理想候选者。
We have sensitively detected DNA hybridization using carbon nanotube field-effect transistors (CNTFETs) in real time. Amino modified peptide nucleic acid (PNA) oligonucleotides at 5' end were covalently immobilized onto the Au surface of the back gate. For 11-mer PNA oligonucletide probe, full-complementary DNA with concentration as low as 6.8 fM solution could be effectively detected. Our CNTFET-based biochip is a promising candidate for the development of an integrated, high-throughput, multiplexed DNA biosensor for medical, forensic and environmental diagnostics.