Promoting Endothelial Cell Affinity and Antithrombogenicity of Polytetrafluoroethylene (PTFE) by Mussel-Inspired Modification and RGD/Heparin Grafting.
Promoting Endothelial Cell Affinity and Antithrombogenicity of Polytetrafluoroethylene (PTFE) by Mussel-Inspired Modification and RGD/Heparin Grafting.
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通过受贻贝启发的修饰和 RGD/肝素移植提高聚四氟乙烯 (PTFE) 的内皮细胞亲和力和抗血栓形成能力
DOI：
10.1039/c8tb00654g
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发表时间：
2018-06-07
期刊：
影响因子：
--
通讯作者：
Turng LS
中科院分区：
文献类型：
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作者：
Mi HY;Jing X;Thomsom JA;Turng LS
当用作小直径血管移植物（SDVG）时，由于缺乏内皮细胞层，合成生物医学材料（如聚四氟乙烯（PTFE））可能会诱导血栓形成和内膜增生。由于其疏水性，PTFE在水溶液中的改性是困难的。本文中，旨在同时促进内皮细胞亲和性和抗血栓形成性，采用贻贝启发的修饰方法来实现各种生物活性分子如RGD和肝素的接枝。这种方法涉及一系列实用的步骤，包括氧等离子体处理，多巴胺（DA）涂层，聚乙烯亚胺（PEI）接枝，和RGD或RGD/肝素固定。通过傅里叶变换红外光谱（FTIR）和X射线光电子能谱（XPS）验证了每一步的成功改性。等离子体处理增加了PTFE的亲水性，从而使其能够有效地涂覆多巴胺。多巴胺，RGD和肝素的接枝导致表面粗糙度的增加和水接触角的减少，由于增加的表面能。多巴胺和RGD修饰后血小板粘附增加，但肝素引入后显著降低。所有这些修饰，特别是掺入RGD，对内皮细胞的附着，活力和增殖表现出良好的效果。由于内皮细胞和RGD之间的强细胞-基质相互作用，RGD/肝素接枝的PTFE表现出高内皮细胞亲和力。这种简单的修饰方法非常适合所有疏水表面，并为SDVG修饰提供了一种有前途的技术，以刺激快速内皮化和有效的抗血栓形成。使用多巴胺、聚乙烯亚胺、RGD和肝素的化学修饰能够同时促进聚四氟乙烯的内皮细胞亲和性和抗血栓形成性。
When used as small-diameter vascular grafts (SDVGs), synthetic biomedical materials like polytetrafluoroethylene (PTFE) may induce thrombosis and intimal hyperplasia due to the lack of an endothelial cell layer. Modification of the PTFE in an aqueous solution is difficult because of its hydrophobicity. Herein, aiming to simultaneously promote endothelial cell affinity and antithrombogenicity, a mussel-inspired modification approach was employed to enable the grafting of various bioactive molecules like RGD and heparin. This approach involves a series of pragmatic steps including oxygen plasma treatment, dopamine (DA) coating, polyethylenimine (PEI) grafting, and RGD or RGD/heparin immobilization. Successful modification in each step was verified via Fourier transform infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS). Plasma treatment increased the hydrophilicity of PTFE, thereby allowing it to be efficiently coated with dopamine. Grafting of dopamine, RGD, and heparin led to an increase in surface roughness and a decrease in water contact angle due to increased surface energy. Platelet adhesion increased after dopamine and RGD modification, but it dramatically decreased when heparin was introduced. All of these modifications, especially the incorporation of RGD, showed favorable effects on endothelial cell attachment, viability, and proliferation. Due to strong cell–substrate interactions between endothelial cells and RGD, the RGD/heparin-grafted PTFE demonstrated high endothelial cell affinity. This facile modification method is highly suitable for all hydrophobic surfaces and provides a promising technique for SDVG modification to stimulate fast endothelialization and effective antithrombosis. Chemical modification using dopamine, polyethylenimine, RGD and heparin enabled simultaneous promotion of endothelial cell affinity and antithrombogenicity of polytetrafluoroethylene.