Bi-directional activation between mesenchymal stem cells and CLL B-cells: implication for CLL disease progression.
Bi-directional activation between mesenchymal stem cells and CLL B-cells: implication for CLL disease progression.
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DOI：
10.1111/j.1365-2141.2009.07868.x
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发表时间：
2009-11
影响因子：
6.5
通讯作者：
Kay NE
中科院分区：
文献类型：
--
作者：
Ding W;Nowakowski GS;Knox TR;Boysen JC;Maas ML;Schwager SM;Wu W;Wellik LE;Dietz AB;Ghosh AK;Secreto CR;Medina KL;Shanafelt TD;Zent CS;Call TG;Kay NE
假设慢性淋巴细胞白血病（CLL）B细胞和骨髓基质细胞之间的接触影响两种细胞类型。为了验证这一假设，我们利用了一个长期的原代培养系统，从骨活检，可靠地产生间充质干细胞（MSC）。MSC与CLL B细胞的共培养保护后者免于自发性凋亡和药物诱导的凋亡。在先前CD 38阳性的CLL B细胞中的CD 38表达在MSC共培养物中上调。在共培养中发现CLL B细胞中CD 71、CD 25、CD 69和CD 70上调。CD 71上调与高危CLL相关性更显著，提示CD 71调节可预测疾病进展。在MSC中，当CLL B细胞和MSC通过transwell分离时，检测到快速ERK和AKT磷酸化（30分钟内）;表明MSC的活化由可溶性因子介导。这些发现支持骨髓基质细胞和CLL B细胞之间的双向激活。
It was hypothesized that contact between chronic lymphocytic leukemia (CLL) B-cells and marrow stromal cells impact both cell types. To test this hypothesis, we utilized a long-term primary culture system from bone biopsies that reliably generates a mesenchymal stem cell (MSC). Co-culture of MSC with CLL B-cells protected the latter from both spontaneous apoptosis and drug-induced apoptosis. The CD38 expression in previously CD38 positive CLL B-cells was up-regulated with MSC co-culture. Up-regulation of CD71, CD25, CD69 and CD70 in CLL B-cells was found in the co-culture. CD71 up-regulation was more significantly associated with high-risk CLL, implicating CD71 regulation in the microenvironment predicting disease progression. In MSC, rapid ERK and AKT phosphorylation (within 30 min) were detected when CLL B-cells and MSC were separated by transwell; indicating that activation of MSC was mediated by soluble factors. These findings support a bi-directional activation between bone marrow stromal cells and CLL B-cells.
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影响因子：
2.1
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