STAR IS REQUIRED FOR NEURONAL DIFFERENTIATION IN THE DROSOPHILA RETINA AND DISPLAYS DOSAGE-SENSITIVE INTERACTIONS WITH RAS 1
STAR IS REQUIRED FOR NEURONAL DIFFERENTIATION IN THE DROSOPHILA RETINA AND DISPLAYS DOSAGE-SENSITIVE INTERACTIONS WITH RAS 1
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DOI：
10.1006/dbio.1993.1285
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发表时间：
1993-11-01
影响因子：
2.7
通讯作者：
RUBIN, GM
中科院分区：
文献类型：
--
作者：
HEBERLEIN, U;HARIHARAN, IK;RUBIN, GM
感光细胞 R8、R2 和 R5 是果蝇眼成象盘中最先启动神经元分化的细胞。我们之前已经表明，这三种细胞需要 Stargene 功能才能进行正确的小眼组装。由于与 Star 突变相关的胚胎致死性，我们分析了马赛克眼成象盘中 Star 功能丧失的后果。假定缺乏Star功能的R8、R2和R5细胞无法分化成神经元并在几小时后死亡。增强子陷阱插入表明，眼盘中的 Star 表达仅限于发育中的 R8、R2 和 R5 细胞。总而言之，这些数据表明 Star 是接收信号和/或执行导致 R8、R2 和 R5 神经元分化的发育程序所必需的。Star 也是翼静脉的形成所必需的，并且在发育中的静脉中表达，这表明在感光细胞分化和翼静脉形成期间可能至少部分重叠的途径起作用。 Starin 细胞间信号传导的作用得到了对 Star 和突变之间遗传相互作用的观察的支持，这些突变减少了通过 Sevenless 和果蝇 EGF 受体同源物（包括 Ras1 和 Son of Sevenless）的信号传导。
The photoreceptor cells R8, R2, and R5 are the first cells to initiate neuronal differentiation in theDrosophilaeye imaginal disc. We have shown previously that these three cells requireStargene function for proper ommatidial assembly. Due to the embryonic lethality associated with mutations inStar, we have analyzed the consequence of loss ofStarfunction in mosaic eye imaginal discs. Presumptive R8, R2, and R5 cells that lackStarfunction fail to differentiate neuronally and die a few hours later. Enhancer trap insertions reveal thatStarexpression in the eye disc is restricted to the developing R8, R2, and R5 cells. Taken together, these data suggest thatStaris required for the reception of a signal and/or the execution of a developmental program that leads to the neuronal differentiation of R8, R2, and R5.Staris also required for the formation of wing veins and is expressed in developing veins, suggesting that at least partially overlapping pathways may operate during photoreceptor cell differentiation and wing vein formation. The role ofStarin cell-cell signaling is supported by the observation of genetic interactions betweenStarand mutations that reduce signaling through bothsevenlessand theDrosophilaEGF-receptor homologue, includingRas1andSon of sevenless.