Performance of viruses and bacteriophages for fecal source determination in a multi-laboratory, comparative study
Performance of viruses and bacteriophages for fecal source determination in a multi-laboratory, comparative study
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DOI：
10.1016/j.watres.2013.04.064
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发表时间：
2013-11-15
期刊：
影响因子：
12.8
通讯作者：
Wicki, Melanie
中科院分区：
文献类型：
--
作者：
Harwood, Valerie J.;Boehm, Alexandria B.;Wicki, Melanie
使用加标到人工淡水中的挑战粪便和污水样本进行了微生物来源追踪（MST）方法准确性的实验室间研究，并将其作为未知样本（盲法供试样本）提供给实验室。源识别协议项目（SIPP）的结果在一系列论文中介绍，涵盖41 MST方法。这篇文章详细介绍了针对人类粪便或污水污染的病毒和噬菌体方法的结果。用作源标识符的人类病毒包括腺病毒（HAdV）、肠道病毒（EV）、诺如病毒I组和II组（诺维和NoVII）以及多瘤病毒（HPyV）。还采用了噬菌体，包括体细胞大肠杆菌和F-特异性RNA噬菌体（FRNAPH）作为粪便污染的一般指标。针对人类粪便来源的噬菌体方法包括FRNAPH分离株的基因分型和细菌宿主屎肠球菌MB-55、拟杆菌HB-73和拟杆菌GB-124上的噬斑形成。小样本量的使用（
An inter-laboratory study of the accuracy of microbial source tracking (MST) methods was conducted using challenge fecal and sewage samples that were spiked into artificial freshwater and provided as unknowns (blind test samples) to the laboratories. The results of the Source Identification Protocol Project (SIPP) are presented in a series of papers that cover 41 MST methods. This contribution details the results of the virus and bacteriophage methods targeting human fecal or sewage contamination. Human viruses used as source identifiers included adenoviruses (HAdV), enteroviruses (EV), norovirus Groups I and II (NoVI and NoVII), and polyomaviruses (HPyVs). Bacteriophages were also employed, including somatic coliphages and F-specific RNA bacteriophages (FRNAPH) as general indicators of fecal contamination. Bacteriophage methods targeting human fecal sources included genotyping of FRNAPH isolates and plaque formation on bacterial hosts Enterococcus faecium MB-55, Bacteroides HB-73 and Bacteroides GB-124. The use of small sample volumes (