Rapid redistribution of glutamate receptors contributes to long-term depression in hippocampal cultures
Rapid redistribution of glutamate receptors contributes to long-term depression in hippocampal cultures
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DOI：
10.1038/8123
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发表时间：
1999-05-01
影响因子：
25
通讯作者：
Malenka, RC
中科院分区：
文献类型：
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作者：
Carroll, RC;Lissin, DV;Malenka, RC
突触强度可以通过各种突触前或突触后修饰来改变。在这里，我们检验了这样的假设：长期抑郁 (LTD) 涉及海马神经元原代培养物中单个突触处聚集的谷氨酸受体数量减少。与海马切片中观察到的 LTD 的突出形式类似，海马培养物中的 LTD 需要 NMDA 受体激活，并伴随着微型兴奋性突触后电流的幅度和频率的降低。免疫细胞化学分析表明，LTD 的诱导导致突触处 AMPA 受体数量同时减少，但对突触 NMDA 受体簇没有影响。这些结果表明，突触谷氨酸受体的亚型特异性重新分布有助于 NMDA 受体依赖性 LTD。
Synaptic strength can be altered by a variety of pre- or postsynaptic modifications. Here we test the hypothesis that long-term depression (LTD) involves a decrease in the number of glutamate receptors that are clustered at individual synapses in primary cultures of hippocampal neurons. Similar to a prominent form of LTD observed in hippocampal slices, LTD in hippocampal cultures required NMDA receptor activation and was accompanied by a decrease in the amplitude and frequency of miniature excitatory postsynaptic currents. Immunocytochemical analysis revealed that induction of LTD caused a concurrent decrease in the number of AMPA receptors clustered at synapses but had no effect on synaptic NMDA receptor clusters. These results suggest that a subtype-specific redistribution of synaptic glutamate receptors contributes to NMDA receptor-dependent LTD.