THE ISOLATION AND CHARACTERIZATION OF PYRIMIDINE-PSORALEN PHOTOADDUCTS FROM DNA
THE ISOLATION AND CHARACTERIZATION OF PYRIMIDINE-PSORALEN PHOTOADDUCTS FROM DNA
复制标题
DNA 中嘧啶-补骨脂素光加合物的分离和表征
DOI：
10.1021/ja00399a035
复制
发表时间：
1980
期刊：
影响因子：
--
通讯作者：
K. Straub
中科院分区：
文献类型：
--
作者：
K. Straub
We have examined the photoadducts of 4'-hydroxymethyl-4,5',8-trimethylpsoralen (HMT) and native DNA. Five nucleoside-HMT monoaddition products have been isolated and characterized, corresponding to three deoxythymidine-HMT and two deoxyuridine (derived from deoxycytidine)-HMT adducts. Structural assignments are based on high resolution mass spectrometry and /sup 1/H NMR studies, including homonuclear spin decoupling and nuclear Overhauser effect (NOE) experiments. The results of this study indicate that (1) a limited number of nucleoside-psoralen adducts are formed with native, double-stranded DNA, and (2) the sterochemistry of the adducts is apparently determined by the geometry of the noncovalent intercalative complex formed by HMT and DNA prior to irradiation. 8 figures, 8 tables.
