Analysis of gene expression using simian virus 40 vectors.
Analysis of gene expression using simian virus 40 vectors.
复制标题
使用猿猴病毒 40 载体分析基因表达。
DOI：
10.1016/0003-2697(83)90723-6
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发表时间：
1983
影响因子：
2.9
通讯作者：
Southern,PJ
中科院分区：
文献类型：
--
作者：
Subramani,S;Southern,PJ
对DNA肿瘤病毒猿猴病毒40（Simian Virus 40，SV40）的广泛研究提供了关于基因组结构、病毒基因表达调控和DNA复制机制的丰富信息。SV40可以在允许的猴细胞中裂解生长，或者病毒DNA可以整合到非允许的啮齿动物细胞的宿主基因组中，引起形态学转化。病毒DNA作为微小染色体存在于裂解感染细胞的细胞核内，并且作为DNA复制的结果，在感染期间存在病毒基因组的显著扩增。这些特性表明，SV40可以被开发为一个转导载体，将外源DNA导入哺乳动物细胞，并在SV40感染周期中表达这种外源DNA。本文对SV40病毒载体和SV40杂合质粒载体的特性进行了描述和比较。
Extensive studies on the DNA tumor virus Simian Virus 40 (SV40) have provided a wealth of information regarding the genome organization, regulation of viral gene expression, and the mechanism of DNA replication. SV40 can grow lytically in permissive monkey cells or the viral DNA can integrate into the host genome of nonpermissive rodent cells causing morphological transformation. The viral DNA exists as a minichromosome within the nuclei of lytically infected cells and, as a consequence of DNA replication, there is a significant amplification of the viral genome during infection. These properties suggested that SV40 could be developed as a transducing vector to introduce exogenous DNA into mammalian cells and to express this foreign DNA during the SV40 infectious cycle. In this article the properties of SV40 virus vectors and SV40 hybrid plasmid vectors are described and contrasted.