Absence of adaptive nonshivering thermogenesis in a marsupial, the fat-tailed dunnart (Sminthopsis crassicaudata)
Absence of adaptive nonshivering thermogenesis in a marsupial, the fat-tailed dunnart (Sminthopsis crassicaudata)
复制标题
有袋动物肥尾袋鼩（Sminthopsis crassicaudata）缺乏适应性非颤抖生热作用
DOI：
--
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Peter B. Frappell
中科院分区：
文献类型：
--
作者：
E. Polymeropoulos;Martin Jastroch;Peter B. Frappell
有袋类动物是否存在非颤抖性产热，一直存在争议。在寒冷的环境中生存的小真兽目物种是至关重要的依赖解偶联蛋白1（UCP1）介导的，自适应nonshriving产热，是在棕色脂肪组织中执行。在一个小的dasyurid有袋类物种，肥尾dunnart（Sminthopsis crassicaudata），UCP1的直向同源物最近已被确定，这是上调在冷暴露类似的自适应分子调整的真兽目棕色脂肪组织。在这里，我们测试了产热功能的有袋动物棕色脂肪组织和UCP1通过评估能力的nonshriving产热冷驯化的dunnarts。在对最佳剂量的去甲肾上腺素的反应中，与热适应的杜那特鱼（24°C）相比，冷适应的杜那特鱼（12°C）没有表现出额外的去甲肾上腺素诱导的最大产热能力的募集。虽然没有观察到驯化组之间的体温差异，但基础代谢率在冷驯化后显著升高。因此，我们认为，自适应nonshriving产热不发生在这个有袋类动物的物种，尽管冷招聘的氧化能力和UCP1在肩胛间脂肪存款。总之，古代UCP直系同源的有袋动物没有贡献的经典nonshriving产热，并可能表现出不同的生理作用。
The presence of nonshivering thermogenesis in marsupials is controversially debated. Survival of small eutherian species in cold environments is crucially dependent on uncoupling protein 1 (UCP1)-mediated, adaptive nonshivering thermogenesis that is executed in brown adipose tissue. In a small dasyurid marsupial species, the fat-tailed dunnart (Sminthopsis crassicaudata), an orthologue of UCP1 has been recently identified which is upregulated during cold exposure resembling adaptive molecular adjustments of eutherian brown adipose tissue. Here, we tested for a thermogenic function of marsupial brown adipose tissue and UCP1 by evaluating the capacity of nonshivering thermogenesis in cold-acclimated dunnarts. In response to an optimal dosage of noradrenaline, cold-acclimated dunnarts (12°C) showed no additional recruitment of noradrenaline-induced maximal thermogenic capacity in comparison to warm-acclimated dunnarts (24°C). While no differences in body temperature were observed between the acclimation groups, basal metabolic rate was significantly elevated after cold acclimation. Therefore, we suggest that adaptive nonshivering thermogenesis does not occur in this marsupial species despite the cold recruitment of oxidative capacity and UCP1 in the interscapular fat deposit. In conclusion, the ancient UCP orthologue in marsupials does not contribute to the classical nonshivering thermogenesis, and may exhibit a different physiological role.
DOI：
10.1073/pnas.96.25.14623
发表时间：
1999-12-07
影响因子：
11.1
作者：
Gavrilova, O;Leon, LR;Reitman, ML
通讯作者：
Reitman, ML