Local ileal cytokine responses in cattle during a primary infection with Cryptosporidium parvum
Local ileal cytokine responses in cattle during a primary infection with Cryptosporidium parvum
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DOI：
10.2307/3284541
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发表时间：
1998-02-01
影响因子：
1.3
通讯作者：
Gasbarre, LC
中科院分区：
文献类型：
--
作者：
Canals, A;Pasquali, P;Gasbarre, LC
在本研究中，本地化的变化，细胞因子的转录谱进行了检查新生小牛后，隐孢子虫的主要感染，采用竞争性逆转录酶聚合酶链反应（RT-PCR）。从感染C.细小病毒感染对含有内部细胞因子基因竞争分子的cDNA样本进行的竞争性RT-PCR显示，LPL和IEL人群中的干扰素-γ和白细胞介素-12（IL-12）（P40）mRNA水平增加，但引流LN中没有。此外，新鉴定的生长因子IL-15的mRNA水平在感染动物的IEL中降低。当分析样品的IL-10时，在任何细胞群体中均未观察到一致的差异，并且在所有3个淋巴细胞群体中，感染组和对照组中IL-2和IL-4的mRNA水平均较低且高度可变。
In the present study, localized changes in cytokine transcription profiles were examined in neonatal calves following a primary infection with Cryptosporidium parvum, using competitive reverse transcriptase polymerase chain reaction (RT-PCR). Total RNA was prepared from ileocecal lymph nodes (LN), lamina propria lymphocytes (LPL), and intraepithelial lymphocytes (IEL) isolated from neonatal calves 7 days after C. parvum infection. Competitive RT-PCR performed on cDNA samples containing internal cytokine gene competitor molecules showed increases in the levels of interferon-gamma and interleukin-12 (IL-12) (P40) mRNA in both LPL and IEL populations but not in the draining LN. In addition, the levels of mRNA of the newly identified growth factor IL-15 decreased in the IEL of the infected animals. No consistent differences were seen in any of the cell populations when the samples were analyzed for IL-10 and levels of mRNA for IL-2 and IL-4 were low and highly variable in both infected and control groups in all 3 lymphocyte populations.