A high-density linkage map of Theobroma cacao L.
A high-density linkage map of Theobroma cacao L.
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DOI：
10.1007/s001220051566
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发表时间：
2000-10-01
影响因子：
5.4
通讯作者：
Lanaud, C
中科院分区：
文献类型：
--
作者：
Risterucci, AM;Grivet, L;Lanaud, C
Lanaud等（1995）建立的第一个连锁图被用作产生高密度分子连锁图的起点。一个作图群体的181个后代之间的杂交产生的两个杂合基因型，一个Foraslavia和Trinitario（Foraslavia和Criollo之间的杂交），用于连锁分析。建立了一种新的DNA提取方法，该方法允许足够的高质量DNA用于构建基于PCR标记的遗传图谱。该图谱包括424个标记，标记之间的平均间距为2.1 cM。所用标记类型包括5种同工酶、6个已知功能基因位点、65个基因组RFLP、104个cDNA RFLP、3个端粒探针、30个RAPD、191个AFLP和20个微卫星。新的标记类型，AFLP和微卫星的使用，并没有扰乱原来的顺序RFLP位点上使用的前一张地图。遗传标记分布在10个连锁群上，总长度为885.4cM。相邻标记间的最大距离为16.2 cM，9.4%的位点表现出偏分离。
The first linkage map established by Lanaud et al. (1995) was used as a starting point to produce a high-density molecular linkage map. A mapping population of 181 progenies resulting from a cross between two heterozygous genotypes, a Forastero and a Trinitario (hybrid between Forastero and Criollo), was used for the linkage analysis. A new DNA isolation protocol was established, which allows enough good quality DNA to construct a genetic map with PCR-based markers. The map comprises 424 markers with an average spacing between markers of 2.1 cM. The marker types used were five isozymes, six loci from known function genes, 65 genomic RFLPs, 104 cDNA RFLPs, three telomeric probes, 30 RAPDs, 191 AFLPs and 20 microsatellites. The use of new marker types, AFLP and microsatellites, did not disturb the original order of the RFLP loci used on the previous map. The genetic markers were distributed over ten linkage groups and cover 885.4 cM. The maximum distance observed between adjacent markers was 16.2 cM, and 9.4% of all loci showed skewed segregation.