Antigen-presenting cells in parotid glands contain cystatin D originating from acinar cells.
Antigen-presenting cells in parotid glands contain cystatin D originating from acinar cells.
复制标题
腮腺中的抗原呈递细胞含有源自腺泡细胞的半胱氨酸蛋白酶抑制剂 D。
DOI：
10.1016/j.abb.2012.12.009
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Shimomura H
中科院分区：
文献类型：
--
作者：
Nashida T;Sato R;Haga-Tsujimura M;Yoshie S;Yoshimura K;Imai A;Shimomura H
由Cst5编码的胱抑素D是唾液分类的II型胱抑素。我们通过检测胱抑素D蛋白和mRNA在大鼠体内的分布来研究胱抑素D的动态，以发现其新的功能。在腮腺中观察到Cst5和胱抑素D的同时表达，但原位杂交显示只有腺泡细胞产生胱抑素D。合成的胱抑素D定位于小泡中，从顶侧分泌到唾液，从基底外侧分泌到细胞外区域，这是胱抑素D的第二分泌途径。我们还在腮腺中发现了含有胱抑素D但不表达Cst5的抗原提呈细胞。表明从细胞外区域摄取胱抑素D。在血清和肾小管细胞中检测到胱抑素D，表明胱抑素D在体内循环并被肾小管细胞摄取。因此，揭示了胱抑素D的新动态，并提出了胱抑素D在调节抗原呈递细胞活性中的作用。
Cystatin D encoded by Cst5 is a salivary classified type II cystatin. We investigated the dynamism of cystatin D by examining the distribution of cystatin D protein and mRNA in rats, to identify novel functions. The simultaneous expression of Cst5 and cystatin D was observed in parotid glands, however in situ hybridization showed that only acinar cells produced cystatin D. Synthesized cystatin D was localized in small vesicles and secreted from the apical side to the saliva, and from the basolateral side to the extracellular region, a second secretory pathway for cystatin D. We also identified antigen-presenting cells in the parotid glands that contained cystatin D without the expression of Cst5, indicating the uptake of cystatin D from the extracellular region. Cystatin D was detected in blood serum and renal tubular cells with megalin, indicating the circulation of cystatin D through the body and uptake by renal tubular cells. Thus, the novel dynamism of cystatin D was shown and a function for cystatin D in the regulation of antigen-presenting cell activity was proposed.