Light-induced nuclear export reveals rapid dynamics of epigenetic modifications.
Light-induced nuclear export reveals rapid dynamics of epigenetic modifications.
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DOI：
10.1038/nchembio.2068
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发表时间：
2016-06
影响因子：
14.8
通讯作者：
Kuhlman B
中科院分区：
文献类型：
--
作者：
Yumerefendi H;Lerner AM;Zimmerman SP;Hahn K;Bear JE;Strahl BD;Kuhlman B
我们设计了一种光活化系统，通过将核输出信号嵌入来自光致蛋白1的LOV2结构域中，快速可逆地从细胞核输出蛋白质。将染色质修饰剂Bre1与光开关融合，我们实现了酵母中组蛋白H2B单泛素化的光依赖性控制，揭示了泛素标记的快速周转。此外，这种诱导系统使我们能够动态监测依赖于H2B泛素化的表观遗传修饰的状态。
We engineered a photoactivatable system for rapidly and reversibly exporting proteins from the nucleus by embedding a nuclear export signal in the LOV2 domain from phototropin 1. Fusing the chromatin modifier Bre1 to the photoswitch, we achieved light-dependent control of histone H2B monoubiquitylation in yeast, revealing fast turnover of the ubiquitin mark. Moreover, this inducible system allowed us to dynamically monitor the status of epigenetic modifications dependent on H2B ubiquitylation.