The circadian clock network in the brain of different Drosophila species
The circadian clock network in the brain of different Drosophila species
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DOI：
10.1002/cne.23178
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发表时间：
2013-02-01
影响因子：
2.5
通讯作者：
Helfrich-Foerster, Charlotte
中科院分区：
文献类型：
--
作者：
Hermann, Christiane;Saccon, Rachele;Helfrich-Foerster, Charlotte
对细胞和分子生物钟机制的比较研究揭示了不同动物群体之间生物钟组织的惊人相似性。为了深入了解果蝇属内时钟网络的特性，我们分析了时钟蛋白同源物的序列同一性和相似性，并使用抗vrille（VRI），PAR蛋白结构域1（PDP1）和隐花色素（CRY）的抗体对10种不同果蝇进行免疫染色。我们发现，这两个亚属Sophophora和果蝇的时钟网络包括所有的侧和背时钟神经元簇，以前描述的果蝇。然而，对CRY和神经肽色素分散因子（PDF）的免疫染色显示了种属特异性差异。果蝇亚属和D.在大的腹外侧时钟神经元（ILNvs）中，Sophophora亚属的pseudobscura完全缺乏CRY，并且在小的腹外侧时钟神经元（sLNvs）中显示减少的PDF免疫染色。与此相反，我们发现的离子转运肽（ITP）的表达是一致的第五sLNv和一个背外侧时钟神经元（LNd）在所有调查的物种，这表明一个保守的推定功能，这种神经肽的时钟。我们的结论是，时钟网络的一般解剖是高度保守的整个果蝇属，虽然有PDF和CRY表达的变化。我们的比较研究是了解适应不同栖息地的果蝇生物钟组织的第一步。J.Comp.神经元521：367388，2013. (c)2012 Wiley Periodicals，Inc.
Comparative studies on cellular and molecular clock mechanisms have revealed striking similarities in the organization of the clocks among different animal groups. To gain evolutionary insight into the properties of the clock network within the Drosophila genus, we analyzed sequence identities and similarities of clock protein homologues and immunostained brains of 10 different Drosophila species using antibodies against vrille (VRI), PAR-protein domain1 (PDP1), and cryptochrome (CRY). We found that the clock network of both subgenera Sophophora and Drosophila consists of all lateral and dorsal clock neuron clusters that were previously described in Drosophila melanogaster. Immunostaining against CRY and the neuropeptide pigment-dispersing factor (PDF), however, revealed species-specific differences. All species of the Drosophila subgenus and D. pseudoobscura of the Sophophora subgenus completely lacked CRY in the large ventrolateral clock neurons (lLNvs) and showed reduced PDF immunostaining in the small ventrolateral clock neurons (sLNvs). In contrast, we found the expression of the ion transport peptide (ITP) to be consistent within the fifth sLNv and one dorsolateral clock neuron (LNd) in all investigated species, suggesting a conserved putative function of this neuropeptide in the clock. We conclude that the general anatomy of the clock network is highly conserved throughout the Drosophila genus, although there is variation in PDF and CRY expression. Our comparative study is a first step toward understanding the organization of the circadian clock in Drosophila species adapted to different habitats. J. Comp. Neurol. 521:367388, 2013. (c) 2012 Wiley Periodicals, Inc.