Plate height determination for gradient elution chromatography of proteins.
Plate height determination for gradient elution chromatography of proteins.
复制标题
蛋白质梯度洗脱色谱的板高度测定。
DOI：
10.1002/bit.260480506
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发表时间：
1995
影响因子：
3.8
通讯作者：
S. Yamamoto
中科院分区：
文献类型：
--
作者：
S. Yamamoto
提出了一种由蛋白质的线性梯度洗脱(LGE)离子交换层析(IEC)得到的洗脱曲线确定平板高度HETP的方法。该方法基于层析模型的数值解，该模型将区域锐化和分配系数视为盐浓度的函数。极板高度HETP由LGE中峰的宽度和洗脱峰的盐浓度确定。将该方法应用于不同离子交换介质的实验结果。给出了一个基于本方法的计算实例，说明了如何调整色层参数和操作参数以获得所需的分辨率。文中还给出了一种简化的峰形计算方法。(C)1995 John Wiley&Sons，Inc.
A method for determining the plate height HETP from the elution curve obtained by the linear gradient elution (LGE) ion-exchange chromatography (IEC) of proteins is presented. The method was developed on the basis of the numerical solutions of a chromatography model which considers the zone sharpening and the distribution coefficient as a function of the salt concentration. The plate height HETP is determined from the peak width and the salt concentration at which the peak is eluted in LGE. The method was applied to the experimental results with various ion-exchange chromatography media. A calculation example based onthe present method is presented to show how the chromatographic and operating parameters should be tuned to obtain a desired resolution. A simplified calculation procedure for the peak profile is also described. (c) 1995 John Wiley & Sons, Inc.