Molecular characterization of Atractolytocestus sagittatus (Cestoda: Caryophyllidea), monozoic parasite of common carp, and its differentiation from the invasive species Atractolytocestus huronensis
Molecular characterization of Atractolytocestus sagittatus (Cestoda: Caryophyllidea), monozoic parasite of common carp, and its differentiation from the invasive species Atractolytocestus huronensis
复制标题
DOI：
10.1007/s00436-011-2673-0
复制
发表时间：
2012-05
影响因子：
2
通讯作者：
E. Bazsalovicsová;I. Králová-Hromadová;J. Štefka;T. Scholz
中科院分区：
文献类型：
--
作者：
E. Bazsalovicsová;I. Králová-Hromadová;J. Štefka;T. Scholz
本文报道了一种寄生于鲤鱼（Cyprinus carpio carpioL.）的单生绦虫-箭形绦虫（Tapetolytocestus sagittatus，Kulakovskaya et Akhmerov，1965）的核糖体DNA区的完整内转录间隔区1和2（ITS 1和ITS 2）以及线粒体细胞色素氧化酶亚基I（cox 1）基因的部分序列。在两个核糖体间隔区中观察到个体内序列多样性。在ITS 1中，总共19个重组克隆产生了8种不同的序列类型（成对序列同一性，99.7-100%），然而，它们并不类似于不同基因组内ITS拷贝（旁系同源物）的典型结构。多态性显示了几个单核苷酸突变目前专门在单克隆，但短的重复基序的数量变化没有观察到。在ITS 2中，总共21个重组克隆产生了10种不同的序列类型（成对序列同一性，97.5-100%）。它们的主要特征是不同数量的（TCGT）n重复序列，导致ITS 2序列分为两个序列变体，这反映了ITS旁系同源物的特异性结构。第三个DNA区域的分析表明，在所有研究的A中均检测到Alcox 1基因（669 bp）的100%相同。射手座个体。比较A.与入侵鲤鱼的寄生虫胡氏溶囊绦虫（A. huronensisAnthony，1958）的比较表明，两种核糖体间隔区的种间差异（ITS 1为81.4-82.5%，ITS 2为74.4-75.2%）以及绦虫1的种内差异均显著超过种内差异，这证实了寄生在同一鱼类宿主上的两种同源绦虫的有效性。
Sequence structure of complete internal transcribed spacer 1 and 2 (ITS1 and ITS2) of the ribosomal DNA region and partial mitochondrial cytochromecoxidase subunit I (cox1) gene sequences were studied in the monozoic tapewormAtractolytocestus sagittatus(Kulakovskaya et Akhmerov, 1965) (Cestoda: Caryophyllidea), a parasite of common carp (Cyprinus carpio carpioL.). Intraindividual sequence diversity was observed in both ribosomal spacers. In ITS1, a total number of 19 recombinant clones yielded eight different sequence types (pairwise sequence identity, 99.7–100%) which, however, did not resemble the structure typical for divergent intragenomic ITS copies (paralogues). Polymorphism was displayed by several single nucleotide mutations present exclusively in single clones, but variation in the number of short repetitive motifs was not observed. In ITS2, a total of 21 recombinant clones yielded ten different sequence types (pairwise sequence identity, 97.5–100%). They were mostly characterized by a varying number of (TCGT)nrepeats resulting in assortment of ITS2 sequences into two sequence variants, which reflected the structure specific for ITS paralogues. The third DNA region analysed, mitochondrialcox1 gene (669 bp) was detected to be 100% identical in all studiedA. sagittatusindividuals. Comparison of molecular data onA. sagittatuswith those onAtractolytocestus huronensisAnthony, 1958, an invasive parasite of common carp, has shown that interspecific differences significantly exceeded intraspecific variation in both ribosomal spacers (81.4–82.5% in ITS1, 74.4–75.2% in ITS2) as well as in mitochondrialcox1, which confirms validity of both congeneric tapeworms parasitic in the same fish host.