Coxsackievirus and adenovirus receptor-positive cells compose the putative stem/progenitor cell niches in the marginal cell layer and parenchyma of the rat anterior pituitary
Coxsackievirus and adenovirus receptor-positive cells compose the putative stem/progenitor cell niches in the marginal cell layer and parenchyma of the rat anterior pituitary
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DOI：
10.1007/s00441-013-1713-8
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发表时间：
2013-12-01
影响因子：
3.6
通讯作者：
Kato, Yukio
中科院分区：
文献类型：
--
作者：
Chen, Mo;Kato, Takako;Kato, Yukio
脑下垂体是一种缓慢的生殖组织，但通过改变体内平衡来积极响应需求。面向残留腔的边缘细胞层（MCL）长期以来被认为是垂体的干/祖细胞龛。另一方面，柯萨奇病毒和腺病毒受体（CAR），其定位于极化上皮的紧密连接处，已知参与特定组织的发育、分化和再生。本研究试图通过CAR免疫组织化学的方法来表征垂体发育过程中MCL的细胞。因此，我们发现CAR定位于大鼠胚胎第（E）11.5天的内陷口腔上皮中面向口腔的单细胞层的顶端表面。在E13.5，当该单层在垂体原基Rathke囊中构建MCL时，CAR阳性细胞占据MCL，并且CAR的这种定位模式在整个生命中持续保持。此外，在实质中还发现CAR阳性细胞簇。CAR阳性细胞对干/祖细胞标志物性别决定区Y盒2（SOX 2）和上皮钙依赖性粘附（E-cadherin）呈阳性。然而，在出生后生长波之前，基底外侧表面的CAR阳性细胞在MCL下方构建了多个细胞层，并且通过SOX 2和E-钙粘蛋白的褪色发生了向假定的迁移细胞表型的细胞类型转变，这表明在实质中存在新的假定小生境的组成。这些数据与我们以前的报道一起表明，CAR阳性细胞是垂体干/祖细胞，并在MCL和实质中构成假定的干/祖细胞龛。
The pituitary gland is a slow generative tissue but actively responds to demands by changing homeostasis. The marginal cell layer (MCL) facing the residual lumen has long been indicated as a stem/progenitor cell niche of the pituitary. On the other hand, the coxsackievirus and adenovirus receptor (CAR), which localizes at the tight-junction of the polarized epithelium, is known to participate in the development, differentiation and regeneration of specified tissues. The present study attempts to characterize the cells lining the MCL during pituitary development by immunohistochemistry of CAR. Consequently, we found that CAR localizes in an apical surface of the single cell layer facing the oral cavity in the invaginating oral epithelium on rat embryonic day (E) 11.5. On E13.5, when this single layer constructs the MCL in the pituitary primordium Rathke's pouch, CAR-positive cells occupied the MCL and this localization pattern of CAR was persistently maintained throughout life. Moreover, clusters of CAR-positive cells were also found in the parenchyma. CAR-positive cells were positive for stem/progenitor cell markers sex-determining region Y-box 2 (SOX2) and epithelial calcium-dependent adhesion (E-cadherin). However, prior to the postnatal growth wave, cells positive for CAR in the basolateral surface constructed multiple cell layers beneath the MCL and cell-type transition to a putative migratory cell phenotype by fading of SOX2 and E-cadherin occurred, suggesting the composition of new putative niches in the parenchyma. These data, together with our previous reports, suggest that CAR-positive cells are pituitary stem/progenitor cells and compose putative stem/progenitor cell niches in the MCL and parenchyma.