Cancer Stem Cells Persist in Many Cancer Cell Lines
Cancer Stem Cells Persist in Many Cancer Cell Lines
复制标题
DOI：
10.4161/cc.3.4.795
复制
发表时间：
2004-02
期刊：
影响因子：
4.3
通讯作者：
T. Setoguchi;T. Taga;T. Kondo
中科院分区：
文献类型：
--
作者：
T. Setoguchi;T. Taga;T. Kondo
干细胞和癌细胞都被认为能够无限增殖。然而，奇怪的是，一些癌症似乎含有干细胞（癌症干细胞）。为了帮助解决这一矛盾，我们研究了已建立的恶性细胞系（已在培养中维持多年）是否含有干细胞亚群。我们已经表明，四种癌细胞系含有一个小的侧群（SP），在许多正常组织中，该侧群富集了组织的干细胞。我们还表明，SP细胞在C6神经胶质瘤细胞系，但不是非SP细胞，可以产生SP和非SP细胞在培养中，并在很大程度上负责该细胞系的体内恶性。我们提出，许多癌细胞系含有少量的干细胞亚群，这些干细胞富含SP，可以无限期地保持在培养中，并且对它们的恶性程度至关重要。
Both stem cells and cancer cells are thought to be capable of unlimited proliferation. Paradoxically, however, some cancers seem to contain stem-like cells (cancer stem cells). To help resolve this paradox, we investigated whether established malignant cell lines, which have been maintained over years in culture, contain a subpopulation of stem cells. We have shown that four cancer cell lines contain a small side population (SP), which, in many normal tissues, is enriched for stem cells of the tissue. We have also shown that SP cells in C6 glioma cell line, but not non-SP cells, can generate both SP and non-SP cells in culture and are largely responsible for the in vivo malignancy of this cell line. We propose that many cancer cell lines contain a minor subpopulation of stem cells that is enriched in a SP, can be maintained indefinitely in culture, and is crucial for their malignancy.