Comparison of immunoglobulin heavy chain isotype expression in Peyer's patch and splenic B-cells.
Comparison of immunoglobulin heavy chain isotype expression in Peyer's patch and splenic B-cells.
复制标题
派尔氏集结和脾 B 细胞中免疫球蛋白重链同种型表达的比较。
DOI：
10.1016/0161-5890(86)90094-5
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发表时间：
1986
影响因子：
3.6
通讯作者：
G. Woloschak
中科院分区：
文献类型：
--
作者：
G. Woloschak
过去的工作表明，来自派尔集合淋巴结（PP）和脾脏的B淋巴细胞在以下方面彼此不同：（1）PP富含IgA产生前体细胞，而脾脏B细胞是IgM前体细胞的丰富来源;（2）与脾脏相比，PP是抗体分泌细胞的不良来源。造成这些差异的原因尚不清楚。在这项工作中，B细胞已被检查表达新再生的表面免疫球蛋白重链在小鼠PP和脾脏的免疫球蛋白同种型表达。双色免疫荧光显示PP中再生两种表面同种型的B细胞水平（IgM+IgG+= 14.9%; IgM+伊加+= 9.4%）高于脾脏中的水平（IgM+IgG+= 0.4%; IgM+伊加+= 0.2%）。从这些B细胞中纯化Poly A+RNA，并通过DNA过量狭缝印迹杂交比较免疫球蛋白重链同种型表达。脾B细胞每μg多聚核糖体RNA中含有较高量（至少两倍）的IG重链特异性mRNA。派尔集合淋巴结B细胞的μ：α比值比脾B细胞稍低，RNA的体外翻译表明，PP B细胞中可翻译的α特异性Poly A+RNA水平高于MLN或脾B细胞;此外，脾B细胞RNA中可翻译的μ-RNA水平高于其他组织。来自这些组织的RNA的北方印迹分析鉴定了主要的μ-、γ2b-和α-杂交序列，尽管与脾来源的B细胞制备物相比，PP来源的B细胞制备物显示γ2b和α的膜形式mRNA富集。这些结果表明PP B细胞（能够再生表面IG）的分化水平可能与脾B细胞的分化水平显著不同。
Past work has shown that B lymphocytes from Peyer's patches (PP) and from spleen differ from each other in the following ways: (1) PP are enriched for IgA-producing precursor cells while splenic B-cells are a rich source of IgM-precursor cells; and (2) PP are a poor source of antibody-secreting cells when compared to the spleen. The reasons for these differences are unclear. In this work B-cells have been examined expressing newly regenerated surface immunoglobulin heavy chains in murine PP and spleens for immunoglobulin isotype expression. Dual color immunofluorescence demonstrated higher levels of B-cells regenerating two surface isotypes in the PP (IgM+IgG+= 14.9%; IgM+IgA+= 9.4%) than in the spleen (IgM+IgG+= 0.4%; IgM+IgA+= 0.2%). Poly A+RNA was purified from these B-cells and compared for immunoglobulin heavy chain isotype expression by DNA-excess slot blot hybridization. Splenic B-cells contained higher amounts (at least two-fold) of Ig heavy chain-specific mRNA per μg of polysomal RNA than did PP B-cells. Peyer's patches B-cells demonstrated slightly lower μ:α ratios than splenic B-cells.In vitrotranslation of the RNAs suggested higher levels of translatable α-specific Poly A+RNA in PP B-cells than in B-cells from MLN or spleen; furthermore, splenic B-cell RNA contained higher levels of translatable μ-rna than did the other tissues examined. Northern blot analysis of RNA derived from these tissues identified major μ-, γ2b-, and α-hybridizing sequences, though PP-derived B-cell preparations were shown to be enriched for the membrane forms of mRNA for γ2b and α when compared to the spleen-derived B-cell preparations. These results suggest that the level of differentiation of PP B-cells (that are capable of regeneration of surface Ig) may differ significantly from that of splenic B-cells.
鼠免疫球蛋白α基因表达来自独特膜外显子的多个转录物。
DOI：
10.1002/j.1460-2075.1983.tb01518.x
发表时间：
1983
期刊：
The EMBO journal
影响因子：
--
作者：
Word,CJ;Mushinski,JF;Tucker,PW
通讯作者：
Tucker,PW
肠道相关免疫系统的免疫学和分子生物学。
影响因子：
1.3
作者：
Woloschak,GE;Tomasi,TB
通讯作者：
Tomasi,TB