AN IMMUNOHISTOCHEMICAL STUDY OF NEURONAL POPULATIONS CONTAINING NEUROPEPTIDES OR GAMMA-AMINOBUTYRATE WITHIN THE SUPERFICIAL LAYERS OF THE RAT DORSAL HORN
AN IMMUNOHISTOCHEMICAL STUDY OF NEURONAL POPULATIONS CONTAINING NEUROPEPTIDES OR GAMMA-AMINOBUTYRATE WITHIN THE SUPERFICIAL LAYERS OF THE RAT DORSAL HORN
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DOI：
10.1016/0306-4522(81)90029-4
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发表时间：
1981-01-01
期刊：
影响因子：
3.3
通讯作者：
WU, JY
中科院分区：
文献类型：
--
作者：
HUNT, SP;KELLY, JS;WU, JY
免疫组织化学技术被用来表征一些interneuronal类型内发现的大鼠背角的浅层。物质P，甲硫氨酸脑啡肽，神经降压素，禽胰多肽，生长抑素和谷氨酸脱羧酶样免疫反应被发现在离散的群体内的细胞体层内的第一至第三层的脊髓后秋水仙碱预处理。每种免疫反应细胞类型在终末分布、核周大小和在背角内的位置方面是不同的。含有P物质的细胞体主要是在第一，第二和第三层，而手术隔离的背角，以消除终端的外在来源表明，终端的本地P物质含有中间神经元存在于整个第一和第二层。脑啡肽免疫反应阳性神经元在第II层内最多，在第I层中较少观察到较大的脑啡肽免疫反应阳性神经元。含神经紧张素的神经元被发现在一个相当狭窄的层沿着II/III边界，与树突arborisations延伸到两个层II和III。神经紧张素免疫反应终末出现在2条带中，一条致密带在第III层内，一条不太致密的带在第I层和第II 0层内。生长抑素样免疫反应被发现在偶尔大的神经元内第三层。禽胰多肽免疫反应定位于神经元内的第一，第二和第三层，是终端免疫反应。含谷氨酸脱羧酶的神经元很大，主要发现于第I、II 0层和II/III边界，其中末端免疫反应性集中。半切结合背根切片表明，这些免疫组织化学可识别的神经元大多数是中间神经元。这些神经元的相互联系和关系，背角投射神经元被认为是在光的镇痛性能的一些这些物质时，直接应用于脊髓。
Immunohistochemical techniques were used to characterize a number of interneuronal types found within the superficial layers of the rat dorsal horn. Substance P, Met-enkephalin, neurotensin, avian pancreatic polypeptide, somatostatin and glutamic acid decarboxylase-like immunoreactivities were found within discrete populations of cell bodies within layers I to III of the spinal cord after colchicine pretreatment. Each immunoreactive cell type was distinct in terms of the distribution of terminals, perikaryal size and position within the dorsal horn. Cell bodies containing substance P were predominantly within layers I, II and III, while surgical isolation of the dorsal horn to remove terminals of extrinsic origin indicated that the terminals of the local substance P-containing interneurons were present throughout layers I and II0. Enkephalin-immunoreactive neurons were most numerous within layer II, with less frequently observed larger enkephalin immunoreactive neurons in layer I. Neurotensin-containing neurons were found in a fairly narrow layer along the II/III border, with dendritic arborisations extending into both layers II and III. Neurotensin-immunoreactive terminals occurred in 2 bands, a dense band within layer IIi and a less dense one within layers I and II0. Somatostatin-like immunoreactivity was found in occasional large neurons within layer IIi. Avian pancreatic polypeptide immunoreactivity was localized to neurons within layers I, II and III, as was terminal immunoreactivity. Glutamic acid decarboxylase-containing neurons were large and were found predominantly within layers I, II0 and on the II/III border, where terminal immunoreactivity was concentrated. Hemisection combined with dorsal root section indicated that the majority of these immunohistochemically identifiable neurons were interneurons. The interconnections and relations of these neurons to dorsal horn projection neurons are considered in the light of reported analgesic properties of a number of these substances when applied directly to the spinal cord.