An enzyme-modulated oxygen-producing micro-system for regenerative therapeutics
An enzyme-modulated oxygen-producing micro-system for regenerative therapeutics
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DOI：
10.1016/j.ijpharm.2011.02.041
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发表时间：
2011-05-16
影响因子：
5.8
通讯作者：
Lim, Jeong Ok
中科院分区：
文献类型：
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作者：
Abdi, Syed Izhar Haider;Ng, Sing Muk;Lim, Jeong Ok
这项研究提出了在生物环境中将氧气视为药物的想法，并表明它将呈现剂量依赖性趋势。为了实现这一点，制造了一个微型系统，具有过氧化氢作为氧气产生源，使用过氧化氢酶分解，过氧化氢酶是几乎所有生物体中发现的一种常见酶。在良好控制和固定条件下，使用细胞活力试验证明了拟定微系统的相关性。该研究在两种受控条件下进行，常氧和缺氧，并且在每种条件下使用三种不同配置的样品进行测试：直接添加H2O2、单层封装的H2O2和双层封装的H2O2。这项研究表明，设计优雅的微系统能够控制H2O2的分解，避免与细胞直接接触，同时在低氧环境下保持细胞活力。(C)2011 Elsevier B.V.保留所有权利。
This study suggests the idea of treating oxygen as a drug in a biological environment and demonstrates that it will exhibit a dosage-dependent trend. To accomplish this, a micro-system was fabricated, having hydrogen peroxide as the oxygen-generating source, which was decomposed using catalase, a common enzyme found in nearly all living organisms. The relevance of the proposed micro-system was justified using cell viability assays under well-controlled and fixed conditions. This study was performed under two controlled conditions, normoxia and hypoxia, and tests were carried out using three different configurations of samples under each condition: direct addition of H2O2, H2O2 encapsulated with single layer, and H2O2 encapsulated with double layers. This study demonstrates that the elegantly designed micro-system managed to control the decomposition of H2O2 and avoided direct contact with cells, while also maintaining cell viability under a low oxygen environment. (C) 2011 Elsevier B.V. All rights reserved.