EXPRESSION OF FACILITATIVE GLUCOSE-TRANSPORTER ISOFORMS IN HUMAN BRAIN-TUMORS
EXPRESSION OF FACILITATIVE GLUCOSE-TRANSPORTER ISOFORMS IN HUMAN BRAIN-TUMORS
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DOI：
10.1111/j.1471-4159.1993.tb07441.x
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发表时间：
1993-12-01
影响因子：
4.7
通讯作者：
HOSHINO, T
中科院分区：
文献类型：
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作者：
NAGAMATSU, S;SAWA, H;HOSHINO, T
检测了人星形细胞肿瘤中易化葡萄糖转运蛋白（GLUT）亚型的表达。使用与人易化葡萄糖转运蛋白家族序列对应的变性寡核苷酸引物对手术活检的胶质母细胞瘤进行逆转录-聚合酶链反应，并将聚合酶链反应产物与人GLUT 1、GLUT 2、GLUT 3、GLUT 4和GLUT 5 cDNA探针杂交。结果显示，活检的胶质母细胞瘤表达GLUT 1、GLUT 3和GLUT 4葡萄糖转运蛋白基因。对活检胶质母细胞瘤的总RNA（10 μ g）的北方印迹分析显示仅GLUT 1和GLUT 3的转录本，表明胰岛素响应性葡萄糖转运蛋白GLUT 4 mRNA的表达相对较低。免疫印迹分析活检的胶质母细胞瘤组织的多克隆抗体对C-末端合成肽的GLUT 1，GLUT 3，和GLUT 4显示一个单一的带的每个多肽。然而，在胶质母细胞瘤中未观察到GLUT 1和GLUT 3葡萄糖转运蛋白的表达升高。还对星形细胞肿瘤组织（n = 14）进行了免疫组化检查。在所有病例中，在毛细血管的管腔表面均观察到GLUT 1反应产物，而14例中仅2例肿瘤细胞GLUT 1阳性。GLUT 3在所有星形细胞瘤细胞中均呈阳性表达。14例中3例表达GLUT 4蛋白，定位于肿瘤细胞胞浆。这些结果表明，促进葡萄糖转运可能会改变星形胶质细胞肿瘤细胞，从而显示在葡萄糖代谢的显着变化。
The expression of facilitative glucose transporter (GLUT) isoforms in human astrocytic tumors was examined. Reverse transcriptase-polymerase chain reaction of a surgically biopsied glioblastoma was carried out using the degenerative oligonucleotide primers corresponding to the sequences of the human facilitative glucose transporter family, and polymerase chain-reaction products were hybridized with human GLUT1, GLUT2, GLUT3, GLUT4, and GLUT5 cDNA probes. The results showed that a biopsied glioblastoma expressed GLUT1, GLUT3, and GLUT4 glucose transporter genes. Northern blot analysis of total RNA (10 mug) from a biopsied glioblastoma showed the transcripts of only GLUT1 and GLUT3, suggesting that the expression of insulin-responsive glucose transporter GLUT4 mRNA is relatively low. Immunoblot analysis of biopsied glioblastoma tissues by polyclonal antibodies against the C-terminal synthetic peptides of GLUT1, GLUT3, and GLUT4 showed a single band of each polypeptide. However, elevated expression of GLUT1 and GLUT3 glucose transporters was not observed in the glioblastoma. Astrocytic tumor tissues (n = 14) were also examined immunohistochemically. Reactive products for GLUT1 were observed in the luminal surface of capillaries in all cases, whereas tumor cells were positive for GLUT1 in only two of 14 cases. GLUT3 was positive in astrocytic tumor cells in all cases. Three of 14 cases expressed the GLUT4 protein, which was localized in the cytoplasm of tumor cells. These results suggest that the facilitative glucose transport may be altered in astrocytic tumor cells and thus display a significant change in glucose metabolism.