A Periplasmic Polymer Curves Vibrio cholerae and Promotes Pathogenesis.
A Periplasmic Polymer Curves Vibrio cholerae and Promotes Pathogenesis.
复制标题
DOI：
10.1016/j.cell.2016.12.019
复制
发表时间：
2017-01-12
期刊：
影响因子：
64.5
通讯作者：
Gitai Z
中科院分区：
文献类型：
--
作者：
Bartlett TM;Bratton BP;Duvshani A;Miguel A;Sheng Y;Martin NR;Nguyen JP;Persat A;Desmarais SM;VanNieuwenhze MS;Huang KC;Zhu J;Shaevitz JW;Gitai Z
致病性霍乱弧菌仍然是一个主要的人类健康问题。胆肠静脉具有特征性的弯曲杆形态，具有较长的外表面和较短的内表面。以前，这种弯曲的机制和功能是未知的。在这里，我们确定和表征CrvA，第一曲率行列式在V. cholesterol。CrvA在细胞弯曲的内表面自组装成细丝。与传统的细胞骨架不同，CrvA定位于周质，因此可以被认为是周质元件。为了量化曲率如何形成，我们开发了QuASAR（球囊结构重塑的定量分析），它测量亚细胞肽聚糖动力学。QuASAR显示CrvA不对称地模式肽聚糖插入而不是去除，导致更多的材料插入到外面而不是里面。此外，crvA是群体调节的，并且在高细胞密度下CrvA依赖性曲率增加。最后，我们证明CrvA促进水凝胶的运动性，并在宿主定植和发病机制方面具有优势。一种周质聚合物减缓了致病性霍乱弧菌一侧的细胞生长，使其呈弯曲形状，促进宿主定植和致病
Pathogenic Vibrio cholerae remains a major human health concern. V. cholerae has a characteristic curved rod morphology, with a longer outer face and a shorter inner face. Previously, the mechanism and function of this curvature were unknown. Here we identify and characterize CrvA, the first curvature determinant in V. cholerae. CrvA self-assembles into filaments at the inner face of cell curvature. Unlike traditional cytoskeletons, CrvA localizes to the periplasm, and thus could be considered a periskeletal element. To quantify how curvature forms, we developed QuASAR (Quantitative Analysis of Sacculus Architecture Remodeling), which measures subcellular peptidoglycan dynamics. QuASAR reveals that CrvA asymmetrically patterns peptidoglycan insertion rather than removal, causing more material insertion into the outer face than the inner face. Furthermore, crvA is quorum regulated and CrvA-dependent curvature increases at high cell density. Finally, we demonstrate that CrvA promotes motility in hydrogels and confers an advantage in host colonization and pathogenesis. A periplasmic polymer slows cell growth on one side of the pathogenic bacterium Vibrio cholera giving it it a curved shape that promotes host colonization and pathogenesis