The von Willebrand factor-binding protein (vWbp) of Staphylococcus aureus is a coagulase
The von Willebrand factor-binding protein (vWbp) of Staphylococcus aureus is a coagulase
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DOI：
10.1016/j.femsle.2004.03.040
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发表时间：
2004-05-15
影响因子：
2.1
通讯作者：
Frykberg, L
中科院分区：
文献类型：
--
作者：
Bjerketorp, J;Jacobsson, K;Frykberg, L
金黄色葡萄球菌编码一种含有482个氨基酸的分泌性血管性血友病因子结合蛋白（vWbp）。该蛋白的n端部分与葡萄凝固酶同源，因此我们研究了vWbp是否具有凝固活性。重组vWbp对人和猪的血浆有较好的凝血效果，但对其他动物的血浆凝血效果较差。凝血效率具有浓度依赖性，可被特异性抗体抑制。此外，vWbp的种特异性凝血作用依赖于与凝血酶原的相互作用。这种相互作用还导致vWbp的特异性裂解，将c端从凝固结构域释放出来。(C) 2004欧洲微生物学会联合会。Elsevier B.V.版权所有。
Staphylococcus aureus encodes a secreted von Willebrand factor-binding protein (vWbp) of 482 amino acids. The N-terminal part of this protein is homologous to staphylocoagulase and therefore we investigated whether vWbp has coagulating activity. Recombinant vWbp was shown to coagulate human and porcine plasma efficiently, but was less active against plasma from other species. The coagulation efficiency was concentration dependent, and could be inhibited by specific antibodies against vWbp. Furthermore, the species-specific coagulation by vWbp depended on the interaction with prothrombin. This interaction also resulted in specific cleavage of vWbp, releasing the C-terminal part from the coagulating domain. (C) 2004 Federation of European Micro biological Societies. Published by Elsevier B.V. All rights reserved.