Reversible cleavage and ligation of hepatitis delta virus RNA.
Reversible cleavage and ligation of hepatitis delta virus RNA.
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丁型肝炎病毒 RNA 的可逆切割和连接。
DOI：
10.1126/science.2492677
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Lai,MM
中科院分区：
文献类型：
--
作者：
Wu,HN;Lai,MM
丁型肝炎病毒RNA的一个148个核苷酸的亚片段被证明可逆地进行切割和连接。反应的方向由Mg2+离子的存在或不存在决定，Mg2+的存在有利于裂解反应。连接需要所涉及的RNA分子的特定构象，并且仅发生在仍然通过氢键保持在一起的两个切割的RNA片段之间。EDTA去除Mg2+后，连接反应迅速发生。这代表了一类新的RNA酶。
A 148-nucleotide subfragment of hepatitis delta virus RNA was shown to undergo cleavage and ligation reversibly. The direction of the reaction is determined by the presence or absence of Mg2+ions, with the presence of Mg2+favoring the cleavage reaction. Ligation requires specific conformation of the RNA molecules involved and occurs only between two cleaved RNA fragments that are still held together by hydrogen bonds. The ligation reaction occurs rapidly on removal of Mg2+by EDTA. This represents a new class of RNA enzymes.