Impact of Introduction of Arbuscular Mycorrhizal Fungi on the Root Microbial Community in Agricultural Fields
Impact of Introduction of Arbuscular Mycorrhizal Fungi on the Root Microbial Community in Agricultural Fields
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DOI：
10.1264/jsme2.me18109
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发表时间：
2019-03-30
影响因子：
2.2
通讯作者：
Sato, Shusei
中科院分区：
文献类型：
--
作者：
Akyol, Turgut Yigit;Niwa, Rieko;Sato, Shusei
丛枝菌根（AM）真菌是根系微生物组的重要成员，可用作可持续农业的生物肥料。为了阐明AM真菌接种对土著根微生物群落的影响，我们使用高通量测序和分析管道提供固定的操作分类单位（OTU）作为输出，以调查在六个农业领域中用商业AM真菌接种物处理的根的细菌和真菌群落。接种AM真菌对各处理根系微生物群落结构均有显著影响。接种改变了土著AM真菌和其他真菌成员的丰富领域依赖的方式。接种通过改变土著细菌的丰度和引入新细菌来持续富集几种细菌OTU。一些接种物相关细菌与引入的AM真菌密切相互作用，其中一些属于伯克霍尔德氏菌属、纤维单胞菌属、微杆菌属、鞘氨醇单胞菌属和链霉菌属，可能是有助于引入的AM真菌的建立和/或功能的候选菌根际细菌。接种的AM真菌也共同发生了几个土著细菌与推定的有益性状，这表明接种的AM真菌可能会招募特定的类群，以赋予更好的植物性能。细菌家族甲基杆菌科、醋杆菌科、Armatimonadaceae和脂环酸杆菌科通过接种持续减少，这可能是由于接种物引起的宿主植物状态的变化。据我们所知，这是第一个大规模的研究，调查AM真菌接种和土著根微生物群落在农业领域之间的相互作用。
Arbuscular mycorrhizal (AM) fungi are important members of the root microbiome and may be used as biofertilizers for sustainable agriculture. To elucidate the impact of AM fungal inoculation on indigenous root microbial communities, we used high-throughput sequencing and an analytical pipeline providing fixed operational taxonomic units (OTUs) as an output to investigate the bacterial and fungal communities of roots treated with a commercial AM fungal inoculum in six agricultural fields. AM fungal inoculation significantly influenced the root microbial community structure in all fields. Inoculation changed the abundance of indigenous AM fungi and other fungal members in a field-dependent manner. Inoculation consistently enriched several bacterial OTUs by changing the abundance of indigenous bacteria and introducing new bacteria. Some inoculum-associated bacteria closely interacted with the introduced AM fungi, some of which belonged to the genera Burkholderia, Cellulomonas, Microbacterium, Sphingomonas, and Streptomyces and may be candidate mycorrhizospheric bacteria that contribute to the establishment and/or function of the introduced AM fungi. Inoculated AM fungi also co-occurred with several indigenous bacteria with putative beneficial traits, suggesting that inoculated AM fungi may recruit specific taxa to confer better plant performance. The bacterial families Methylobacteriaceae, Acetobacteraceae, Armatimonadaceae, and Alicyclobacillaceae were consistently reduced by the inoculation, possibly due to changes in the host plant status caused by the inoculum. To the best of our knowledge, this is the first large-scale study to investigate interactions between AM fungal inoculation and indigenous root microbial communities in agricultural fields.