EARLY IMAGING OF EXPERIMENTAL MYOCARDIAL-INFARCTION BY INTRA-CORONARY ADMINISTRATION OF I-131-LABELLED ANTICARDIAC MYOSIN (FAB')2 FRAGMENTS
EARLY IMAGING OF EXPERIMENTAL MYOCARDIAL-INFARCTION BY INTRA-CORONARY ADMINISTRATION OF I-131-LABELLED ANTICARDIAC MYOSIN (FAB')2 FRAGMENTS
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DOI：
10.1161/01.cir.58.6.1137
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发表时间：
1978-01-01
期刊：
影响因子：
37.8
通讯作者：
HABER, E
中科院分区：
文献类型：
--
作者：
KHAW, BA;GOLD, HK;HABER, E
通过冠状动脉内注射131 I标记的心肌肌球蛋白特异性抗体（Fab“）2，检查心肌梗死早期显像的可行性。12只犬经颈动脉置入球囊导管阻断冠状动脉左前降支5 h。取出导管，将1 mCi的201 Tl和500 μ Ci的131 I抗体静脉注射到主左冠状动脉中。在这些动物中，6只通过ECG和随后的氯化三苯基四氮唑染色显示出心肌梗死的证据，而其他动物则没有。在每只梗塞动物中，注射同位素后1/2小时的体内放射性图显示在心脏的前心尖区中的131 I摄取，对应于201 Tl摄取缺失的区域。这种关系在离体心脏和心脏切片中得到证实。在切片中，131 I摄取对应于未用氯化三苯基四氮唑染色的区域。在6只冠状动脉闭塞后未显示梗死证据的动物中，无论是在体内还是在切除标本中，均未显示131 I摄取。在另外4只接受相同程序的犬中，将来自非免疫IgG [免疫球蛋白G]的125 I标记的（Fab“）2与131 I标记的犬肌球蛋白特异性抗体（Fab "）2同时注射到左主冠状动脉中。梗塞中心与正常组织之间的摄取比率为34.3 ± 0.01。1.5（平均值±）。SEM [平均值的标准误差]），与6.6 ±. 0.4对于非免疫IgG片段，表明冠状动脉内注射不利于梗死区域蛋白质的非特异性隔离。冠状动脉内注射肌球蛋白特异性抗体片段可导致心肌梗死的早期和特异性1/2小时成像。
The feasibility of early imaging of myocardial infarcts by intracoronary injection of 131I-labeled cardiac myosin-specific antibody (Fab'')2 was examined. The left anterior descending coronary artery was occluded for 5 h by a balloon catheter introduced through the carotid artery in 12 dogs. The catheter was withdrawn and 1 mCi 201Tl and 500 .mu.Ci of 131I antibody were injected i.v. into the main left coronary artery. Of these animals, 6 demonstrated evidence of myocardial infarction by ECG and subsequent triphenyl-tetrazolium chloride staining, while the others did not. In each of the infarcted animals, in vivo scintigrams 1/2 h after injection of isotope showed uptake of 131I in the anteroapical region of the heart corresponding to the region of absent 201Tl uptake. This relationship was confirmed in the excised hearts and in heart slices. In slices, 131I uptake corresponded to regions that did not stain with triphenyltetrazolium chloride. In the 6 animals that did not show evidence for infarction after coronary occlusion, uptake of 131I was not demonstrated, either in vivo or in excised specimens. In 4 additional dogs subjected to the same procedure, 125I-labeled (Fab'')2 from nonimmune IgG [immunoglobulin G] was injected simultaneously into the left main coronary artery with 131I-labeled canine myosin-specific antibody (Fab'')2. The ratio of uptake between infarct center and normal tissue was 34.3 .+-. 1.5 (mean .+-. SEM [standard error of the mean]) for the specific antibody fragment as contrasted to 6.6 .+-. 0.4 for the nonimmune IgG fragment, indicating that intracoronary injection does not favor nonspecific sequestration of protein in regions of infarction. The intracoronary administration of myosin-specific antibody fragments leads to early and specific 1/2 h imaging of myocardial infarcts.