An Evaluation of Blood Compatibility of Silver Nanoparticles.
An Evaluation of Blood Compatibility of Silver Nanoparticles.
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DOI：
10.1038/srep25518
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发表时间：
2016-05-05
影响因子：
4.6
通讯作者：
Xie L
中科院分区：
文献类型：
--
作者：
Huang H;Lai W;Cui M;Liang L;Lin Y;Fang Q;Liu Y;Xie L
银纳米颗粒因其优异的抗菌性能在医疗器械中具有巨大的应用潜力。由于潜在的血液接触，应调查AgNPs的血液相容性。然而，到目前为止，大多数研究都不系统，也没有对AgNPs的血液相容性机制提供深入的见解。在这项研究中，我们研究了两种不同表面涂层（聚乙烯醇吡啶酮和柠檬酸盐）的20nm AgNPs的血液生物学效应，包括溶血、淋巴细胞增殖、血小板聚集、凝血和补体活化。我们的研究结果显示，AgNPs在所有研究浓度（10、20、40 μg/mL）下都能引起溶血，并严重影响淋巴细胞的增殖和活力。然而，当浓度达到~40 μg/mL时，AgNPs对血小板聚集、凝血过程或补体激活没有任何影响。AgNPs血浆蛋白冠的蛋白质组学分析表明，酸性和小分子量血浆蛋白优先吸附在AgNPs上，这些蛋白包括一些与止血、凝血、血小板、补体活化和免疫反应相关的重要蛋白。蛋白质组学分析预测AgNPs的生物学效应与我们的实验数据基本一致，因为AgNPs中C3成分较少，涉及血小板聚集和血栓形成的阴性因子多于阳性因子。
Silver nanoparticles (AgNPs) have tremendous potentials in medical devices due to their excellent antimicrobial properties. Blood compatibility should be investigated for AgNPs due to the potential blood contact. However, so far, most studies are not systematic and have not provided insights into the mechanisms for blood compatibility of AgNPs. In this study, we have investigated the blood biological effects, including hemolysis, lymphocyte proliferation, platelet aggregation, coagulation and complement activation, of 20 nm AgNPs with two different surface coatings (polyvinyl pyrrolidone and citrate). Our results have revealed AgNPs could elicit hemolysis and severely impact the proliferation and viability of lymphocytes at all investigated concentrations (10, 20, 40 μg/mL). Nevertheless, AgNPs didn’t show any effect on platelet aggregation, coagulation process, or complement activation at up to ~40 μg/mL. Proteomic analysis on AgNPs plasma proteins corona has revealed that acidic and small molecular weight blood plasma proteins were preferentially adsorbed onto AgNPs, and these include some important proteins relevant to hemostasis, coagulation, platelet, complement activation and immune responses. The predicted biological effects of AgNPs by proteomic analysis are mostly consistent with our experimental data since there were few C3 components on AgNPs and more negative than positive factors involving platelet aggregation and thrombosis.