INTERLEUKIN-1 REGULATES SYNTHESIS OF NERVE GROWTH-FACTOR IN NONNEURONAL CELLS OF RAT SCIATIC-NERVE
INTERLEUKIN-1 REGULATES SYNTHESIS OF NERVE GROWTH-FACTOR IN NONNEURONAL CELLS OF RAT SCIATIC-NERVE
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DOI：
10.1038/330658a0
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发表时间：
1987-12-17
期刊：
影响因子：
64.8
通讯作者：
THOENEN, H
中科院分区：
文献类型：
--
作者：
LINDHOLM, D;HEUMANN, R;THOENEN, H
成年大鼠完整坐骨神经的雪旺细胞和成纤维细胞样细胞合成很少的神经生长因子（NGF）（参考文献1）。然而，损伤后，坐骨神经非神经元细胞合成的NGF-mRNA和NGF蛋白的量显著增加1，2。NGF合成的这种局部增加部分地取代了从外周到NGF响应性感觉和交感神经元的中断的NGF供应，其轴突在坐骨神经内运行1。已知在华勒变性过程中侵入神经损伤部位的巨噬细胞3，4，在调节NGF合成中很重要5。在这里，我们表明，在坐骨神经的培养外植体中的神经生长因子-mRNA水平的巨噬细胞的效果可以模仿由条件培养基的活化巨噬细胞，白细胞介素-1是负责任的代理。
The Schwann cells and fibroblast-like cells of the intact sciatic nerve of adult rats synthesize very little nerve growth factor (NGF) (ref. 1). After lesion, however, there is a dramatic increase in the amounts of both NGF-mRNA and NGF protein synthesized by the sciatic non-neuronal cells1,2. This local increase in NGF synthesis partially replaces the interrupted NGF supply from the periphery to the NGF-responsive sensory and sympathetic neurons, whose axons run within the sciatic nerve1. Macrophages, known to invade the site of nerve lesion during wallerian degeneration3,4, are important in the regulation of NGF synthesis5. Here we demonstrate that the effect of macrophages on NGF-mRNA levels in cultured explants of sciatic nerve can be mimicked by conditioned media of activated macrophages, and that interleukin-1 is the responsible agent.