Characterisation of imidacloprid resistance in the bird cherry-oat aphid, Rhopalosiphum padi, a serious pest on wheat crops
Characterisation of imidacloprid resistance in the bird cherry-oat aphid, Rhopalosiphum padi, a serious pest on wheat crops
复制标题
小麦作物严重害虫鸟樱桃燕麦蚜（Rhopalosiphum padi）对吡虫啉的抗性特征
DOI：
10.1002/ps.4834
复制
发表时间：
2018-06-01
影响因子：
4.1
通讯作者：
Chen, Maohua
中科院分区：
文献类型：
--
作者：
Wang, Kang;Zhang, Meng;Chen, Maohua
禾谷缢管蚜是一种世界性的小麦害虫。研究表明，R. padi已经对包括吡虫啉在内的不同杀虫剂产生了抗药性。本研究从生物化学和分子水平上研究了水稻对吡虫啉的抗性机制。建立了水稻抗吡虫啉（IM-R）品系和敏感（SS）品系。利用生命表进行适合度分析表明，IM-R品系在几个参数上有明显的劣势，表明适合度降低。测定了IM-R和SS对7种杀虫剂的交互抗性。协同和酶活性数据表明，P450在抗性中起作用。此外，细胞色素P450（CYP）基因CYP 6CY 3 -1和CYP 6CY 3 -2的mRNA表达水平在IM-R株中显著增加。在IM-R菌株中未检测到烟碱乙酰胆碱受体（nAChR）亚基内的靶位点突变。有趣的是，nAChR 1，2，3，7-2和1亚基基因的表达水平显著降低，表明这些亚基的下调可能与抗性有关。帕迪。(c)2017年化学工业协会
BACKGROUNDRhopalosiphum padi is a destructive insect pest of wheat worldwide. Studies have shown that R. padi has developed resistance to different insecticides, including imidacloprid. We studied the mechanisms conferring resistance to imidacloprid at the biochemical and molecular levels.RESULTSAn R. padi imidacloprid-resistant (IM-R) strain and a susceptible (SS) strain were established. Fitness analysis using life-tables showed that the IM-R strain had obvious disadvantages in several parameters, indicating reduced fitness. Profiles of cross-resistance of IM-R and SS to seven insecticides were detected. Both synergistic and enzyme activity data suggested that P450 plays a role in resistance. Furthermore, the mRNA expression levels of cytochrome P450 (CYP) genes CYP6CY3-1 and CYP6CY3-2 were significantly increased in the IM-R strain. No target-site mutation within the nicotinic acetylcholine receptor (nAChR) subunits was detected in the IM-R strain. Interestingly, the expression levels of the nAChR 1, 2, 3, 7-2, and 1 subunit genes were significantly decreased, suggesting that down-regulation of these subunits may be involved in resistance.CONCLUSIONMultiple mechanisms confer imidacloprid resistance in R. padi. (c) 2017 Society of Chemical Industry