Recycling endosomes associate with Golgi stacks in sea urchin embryos.
Recycling endosomes associate with Golgi stacks in sea urchin embryos.
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DOI：
10.1080/19420889.2020.1761069
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发表时间：
2020-01-01
影响因子：
--
通讯作者：
Satoh, Akiko K.
中科院分区：
文献类型：
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作者：
Fujii, Syara;Tago, Tatsuya;Satoh, Akiko K.
反式高尔基网络（TGN）和再循环核内体（RE）被认为是分选中心，前者用于新合成的蛋白质，后者用于内吞噬的蛋白质。然而，最近的研究结果表明，TGN也接受内吞物质，RE接受新合成的蛋白，最终到达质膜。最近，我们报道了在果蝇和微管破坏的HeLa细胞中，REs与高尔基体的跨侧有关。REs是高度动态的：它们与高尔基堆栈的分离和关联经常被观察到。重要的是，一种新合成的货物，糖基磷脂酰肌醇锚定的gfp被发现集中在高尔基相关REs （GA-REs）中，而另一种货物VSVG-GFP在高尔基转运到质膜之前被排除在GA-REs中。这表明，货物的分拣发生在高尔基堆和GA-REs的界面。在本研究中，我们证明REs与海胆胚胎中的高尔基堆积有关，进一步表明REs与高尔基堆积的关联在动物界是一种保守的现象。
The trans-Golgi network (TGN) and recycling endosome (RE) have been recognized as sorting centers, the former for newly synthesized and the latter for endocytosed proteins. However, recent findings have revealed that TGN also receives endocytosed materials and RE accepts newly synthesized proteins destined to the plasma membrane. Recently, we reported that in both Drosophila and microtubule-disrupted HeLa cells, REs are associated with the trans-side of Golgi stacks. REs are highly dynamic: their separation from and association with Golgi stacks are often observed. Importantly, a newly synthesized cargo, glycosylphosphatidylinositol-anchored-GFP was found to be concentrated in Golgi-associated REs (GA-REs), while another cargo VSVG-GFP was excluded from GA-REs before post-Golgi trafficking to the plasma membrane. This suggested that the sorting of cargos takes place at the interface of Golgi stacks and GA-REs. In this study, we demonstrated that REs could associate with Golgi stacks in sea urchin embryos, further indicating that the association of REs with Golgi stacks is a well-conserved phenomenon in the animal kingdom.