Abundance and location of DARPP-32 in striato-tegmental circuits of domestic chicks
Abundance and location of DARPP-32 in striato-tegmental circuits of domestic chicks
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DOI：
10.1016/j.jchemneu.2004.05.006
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发表时间：
2004-09-01
影响因子：
2.8
通讯作者：
Csillag, A
中科院分区：
文献类型：
--
作者：
Bálint, E;Kitka, T;Csillag, A
纹状体与脑干多巴胺能核团相连，并接受强多巴胺能输入。本研究利用激光共聚焦扫描显微镜观察了家鸡内侧纹状体（原嗅旁叶）多巴胺能和多巴胺感受性结构的空间关系。我们还分析了腹侧被盖区和黑质的联系。为了标记多巴胺能结构，使用抗酪氨酸羟化酶，并且DARPP-32（多巴胺和cAMP调节的磷蛋白）是多巴胺感受元件的标记物。酪氨酸羟化酶阳性纤维形成篮状并置周围的DARPP-32含有细胞的内侧纹状体。然而，也观察到这样的篮并置DARPP-32免疫阴性细胞。在被盖中，DARPP-32被观察到在经由袢从端脑下降的轴突中。这些静脉曲张的纤维支配腹侧被盖区和黑质，并经常并列多巴胺能神经元和树突。如通过用固蓝逆行追踪所揭示的，大约40%的靶向腹侧被盖的纹状体投射神经元和60%的靶向黑质的纹状体投射神经元对DARPP-32具有免疫反应性。内源性多巴胺可能会产生一个逆行突触的传入纹状体被盖纤维的作用，除了报告的突触外的行动。在鸟类脑干和内侧纹状体中观察到的大量并列证实了相互纹状体-被盖回路的可能性，与行为的强化有关。(C)2004 Elsevier B. V.保留所有权利。
The striatum is reciprocally connected to the brainstem dopaminergic nuclei and receives a strong dopaminergic input. In the present study the spatial relation between the dopaminergic and dopaminoceptive structures of the avian medial striatum (formerly: lobus parolfactorius) was observed by confocal laser scanning microscope in the domestic chick (Gallus domesticus). We also analysed the connections in the area ventralis tegmentalis and the substantia nigra. To label the dopaminergic structures, anti-tyrosine hydroxylase was used and DARPP-32 (dopamine and cAMP regulated phosphoprotein) was a marker of dopaminoceptive elements. The tyrosine hydroxylase positive fibres formed baskets of juxtapositions around the DARPP-32 containing cells of the medial striatum. However, such baskets were also observed to juxtapose DARPP-32 immunonegative cells. In the tegmentum, DARPP-32 was observed in axons descending from the telencephalon via the ansa lenticularis. These varicose fibers innervated the ventral tegmental area and substantia nigra and were often juxtaposed to dopaminergic neurons and dendrites. Approximately 40% of the striatal projection neurons targeting the ventral tegmentum, and 60% of striatal projection neurons targeting the nigra were immunoreactive to DARPP-32, as revealed by retrograde pathway tracing with Fast Blue. Endogenous dopamine may exert a retrograde synaptic effect on the afferent striato-tegmental fibers, apart from the reported extrasynaptic action. The abundance of juxtapositions observed in the avian brainstem, and medial striatum corroborates the possibility of reciprocal striato-tegmental circuits, relevant to the reinforcement of behaviour. (C) 2004 Elsevier B.V. All rights reserved.