HEME IS A POSITIVE REGULATOR OF CYTOCHROME-P-450 GENE-TRANSCRIPTION
HEME IS A POSITIVE REGULATOR OF CYTOCHROME-P-450 GENE-TRANSCRIPTION
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DOI：
10.1016/0003-9861(88)90324-4
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发表时间：
1988-08-01
影响因子：
3.9
通讯作者：
PADMANABAN, G
中科院分区：
文献类型：
--
作者：
BHAT, GJ;PADMANABAN, G
血红素生物合成的抑制剂如CoCl 2、3-氨基-1，2，4-三唑和硫代乙酰胺阻断3-甲基胆蒽介导的细胞色素P-450（c + d）信使RNA的诱导及其在大鼠肝脏中的转录。这种效应是特异性的，因为白蛋白和谷胱甘肽转移酶（Ya + Yc）的信使RNA水平及其转录在血红素耗尽的条件下不会受到显著影响。以非常低的剂量（50 μ g/100 g体重）外源施用血红素能够完全抵消血红素生物合成抑制剂对细胞色素P-450（c + d）信使RNA水平及其转录的影响。这为血红素作为细胞色素P-450基因转录的正调控因子的作用提供了直接证据。
Inhibitors of heme biosynthesis such as CoCl2, 3-amino-1,2,4-triazole, and thioacetamide block the 3-methylcholanthrene-mediated induction of cytochrome P-450 (c + d) messenger RNAs and their transcription in rat liver. This effect is specific, since the messenger RNA levels for albumin and glutathione transferase (Ya + Yc) and their transcription are not significantly influenced under conditions of heme depletion. Exogenous administration of heme at very low doses (50 .mu.g/100 g body wt) is able to completely counteract the heme biosynthetic inhibitors on cytochrome P-450 (c + d) messenger RNA levels and their transcription. This constitutes a direct proof for the role of heme as a positive regulator of cytochrome P-450 gene transcription.