Fluorine analysis of human dentin surrounding resin composite after fluoride application by μ-PIGE/PIXE analysis
Fluorine analysis of human dentin surrounding resin composite after fluoride application by μ-PIGE/PIXE analysis
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DOI：
10.1016/j.nimb.2011.02.063
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发表时间：
2011-10-15
影响因子：
1.3
通讯作者：
Sano, Hidehiko
中科院分区：
文献类型：
--
作者：
Okuyama, Katsushi;Komatsu, Hisanori;Sano, Hidehiko
使用氟化物预防龋齿是基于在氟离子存在下羟基磷灰石转化为氟磷灰石，从而强化牙齿结构。牙本质和树脂复合物（牙齿着色修复材料）的粘附需要牙本质粘结系统，因为树脂复合物不能直接粘附到牙本质。牙本质酸蚀脱矿是牙本质粘接系统中的重要步骤，然而，脱矿也会导致牙齿结构的弱点。如果氟能强化脱矿牙本质，则牙本质-树脂复合体的粘结强度也可能提高。为了验证这一假设，本研究通过（1）拉伸强度测试分析，（2）牙齿结构的SEM分析，（3）利用微质子激发X射线发射（mu-PIXE）和微质子激发γ射线发射（mu-PIGE）探测钙（Ca）和氟（F）的分布模式在高崎高级辐射研究所（TARRI）的用于高级辐射应用的高崎离子加速器（TIARA）中进行的分析。在将树脂复合物与处理的牙本质粘结之前，以两种浓度（0.022%（100 ppm F）和2.21%（10，000 ppm F）NaF溶液）施加氟化物，持续两个时间段（30和60秒）。以相同的方式制备对照，但不施加氟化物。结果表明，10,000 ppm氟处理组的牙本质与树脂界面结合强度高于对照组和100 ppm氟处理组，而10,000 ppm氟处理组的牙本质与树脂界面结合强度高于对照组。此外，PIGE分析显示，在10，000 ppm F样品的混合粘结层中氟浓度较高，表明氟有助于牙本质-树脂复合粘结的强度。混合粘结层内的氟磷灰石的检测表明，粘结强度涉及矿化过程。(C)2011 Elsevier B. V.保留所有权利。
The use of fluoride for the prevention of caries is based on the transformation of hydroxylapatite to fluoroapatite in the presence of fluoride ions, thereby strengthening tooth structure. Adhesion of dentin and resin composite (tooth-colored restoration material) requires a dentin bonding system, since resin composite is not able to adhere to dentin directly. Demineralization of dentin by acid etching is an important step in the dentin bonding system, however, demineralization also introduces weaknesses in tooth structure. If the demineralized dentin could be strengthened by the application of fluoride, then the dentin-resin composite bond strength might also improve. To test this hypothesis, the present study evaluated the influence of fluoride applications on the strength of the dentin-resin composite bond by (1) tensile strength testing analyses, (2) SEM analyses of tooth structure, and (3) detection of calcium (Ca) and fluorine (F) distribution patterns by micro proton-induced X-ray emission (mu-PIXE) and micro proton-induced gamma-ray emission (mu-PIGE) analyses conducted at the Takasaki Ion Accelerators for Advanced Radiation Application (TIARA) at the Takasaki Advanced Radiation Research Institute (TARRI).In this study, the dentin in extracted human molars was exposed by grinding and the dentin was etched with 35% phosphoric acid. Fluoride was applied at two concentrations, 0.022% (100 ppm F) and 2.21% (10,000 ppm F) NaF solution, for two time periods, 30 and 60 s, prior to bonding the resin composite with the treated dentin. Controls were prepared in the same manner, but without the fluoride application. Bond strength was measured with a micro-tensile testing unit, and the fluorine and calcium distributions at the interface between dentin and resin composite were detected by mu-PIGE and mu-PIXE analysis, respectively.Results indicate that the 10,000 ppm F applications resulted in higher bond strengths than observed in either the 100 ppm F applications or the control group. In addition, PIGE analyses showed high concentrations of fluorine in the hybrid bonding layer of the 10,000 ppm F samples, suggesting that the fluorine contributes to the strength of the dentin-resin composite bond. Detection of fluoroapatite within the hybrid bonding layer suggests that bond strength involves remineralization processes. (C) 2011 Elsevier B.V. All rights reserved.