Distribution of Stanniocalcin 1 in Rat Kidney and Its Regulation by Vitamin D3
Distribution of Stanniocalcin 1 in Rat Kidney and Its Regulation by Vitamin D3
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DOI：
10.1159/000052642
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发表时间：
2001-11
影响因子：
--
通讯作者：
K. Ookata;A. Tojo;M. Onozato;M. Kashiwagi;S. Honda;S. Hirose
中科院分区：
文献类型：
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作者：
K. Ookata;A. Tojo;M. Onozato;M. Kashiwagi;S. Honda;S. Hirose
斯钙素是一种糖蛋白激素，首先在鱼类中被描述为低钙因子，最近其哺乳动物对应物已被鉴定。在对照组和1α，25-二羟维生素D3处理的大鼠中确定了斯钙素1的定位及其表达调节。在Henle袢、致密斑细胞、远曲小管（DCT）和皮质集合管（CCD）中检测到斯钙素1的免疫反应性，在髓质集合管中也检测到微弱的免疫反应性。包埋前电镜免疫细胞化学显示，斯钙素1在亨利氏袢、DCT和CCD主细胞的顶膜上。通过1α，25-二羟维生素D3处理，血浆钙升高，斯钙素1的表达增加。总之，斯钙素1在远端肾单位节段的顶膜中表达，并通过维生素D3增强。
Stanniocalcin is a glycoprotein hormone first described in fish as a hypocalcemic factor, and recently its mammalian counterpart has been identified. Localization of stanniocalcin 1 and its regulation of expression were determined in control and 1α,25-dihydroxyvitamin D3-treated rats. Immunoreactivity for stanniocalcin 1 was detected in the loop of Henle, macula densa cells, distal convoluted tubule (DCT), and cortical collecting duct (CCD), and also faintly in the medullary collecting ducts. Pre-embedding electron-microscopic immunocytochemistry revealed stanniocalcin 1 in the apical membrane of cells of loop of Henle, DCT, and principal cells of CCD. The expression of stanniocalcin 1 was increased by elevated plasma calcium via 1α,25-dihydroxyvitamin D3 treatment. In conclusion, stanniocalcin 1 was expressed in the apical membrane of distal nephron segments and enhanced by vitamin D3.