Comparative actinomycete diversity in marine sediments
Comparative actinomycete diversity in marine sediments
复制标题
DOI：
10.3354/ame01211
复制
发表时间：
2008-01-01
影响因子：
1.4
通讯作者：
Jensen, Paul R.
中科院分区：
文献类型：
--
作者：
Prieto-Davo, Alejandra;Fenical, William;Jensen, Paul R.
比较了近海和近海海洋沉积物中培养的放线菌的多样性。测试了菌株的海水对生长的影响，并分析了 16S rRNA 基因序列多样性。总共培养了代表放线菌目 6 个科的 623 株菌株。这些菌株被分为 16 至 63 个操作分类单元 (OTU)，序列同一性范围为 97% 至 100%。大多数 OTU 与之前报道的非海洋来源菌株密切相关（> 98% 序列同一性）；表明大多数不限于海洋。然而，新的 OTU 与之前培养的菌株平均有 96.6% 的序列同一性，并且大约有 96.6% 的序列同一性。三分之一的 OTU 是海洋特有的，这表明沉积物群落包含大量陆地上不存在的放线菌多样性。离岸地点的海洋特异性并未增加，表明距海岸 125 公里范围内陆地影响程度较高。观察到 < 6% 的生长需要海水。的菌株，而 9 个 OTU 的所有成员都具有此特征，揭示了一些谱系之间的高度海洋适应。统计分析预测近海地点的 OTU 多样性更高，并为扩大深海样本探索提供了理论基础。观察到群落组成发生变化，离岸样品中小单孢菌 OTU 的数量增加。 UniFrac（参见 http://bmf2.colorado.edu/unifrac）统计数据支持近海和离岸地点之间的群落组成存在差异。总体而言，176 个菌株中的 123 个具有不同的 16S rRNA 基因序列，表明海洋沉积物中放线菌多样性较高。
The diversity of cultured actinomycete bacteria was compared between near- and offshore marine sediments. Strains were tested for the effects of seawater on growth and analyzed for 16S rRNA gene sequence diversity. In total, 623 strains representing 6 families in the order Actinomycetales were cultured. These strains were binned into 16 to 63 operational taxonomic units (OTUs) over a range of 97 to 100 % sequence identity. The majority of the OTUs were closely related (> 98 % sequence identity) to strains previously reported from non-marine sources; indicating that most are not restricted to the sea. However, new OTUs averaged 96.6 % sequence identity with previously cultured strains and ca. one-third of the OTUs were marine-specific, suggesting that sediment communities include considerable actinomycete diversity that does not occur on land. Marine specificity did not increase at the off-shore sites, indicating high levels of terrestrial influence out to 125 km from shore. The requirement of seawater for growth was observed among < 6%. of the strains, while all members of 9 OTUs possessed this trait, revealing a high degree of marine adaptation among some lineages. Statistical analyses predicted greater OTU diversity at the off-shore sites and provided a rationale for expanded exploration of deep-sea samples. A change in community composition was observed, with the number of Micromonospora OTUs increasing in the off-shore samples. UniFrac (see http://bmf2.colorado.edu/unifrac) statistics support a difference in community composition between near- and off-shore locations. Overall, 123 of 176 strains had distinct 16S rRNA gene sequences, indicating a high level of actinomycete diversity in marine sediments.