Identification of a simple sequence polymorphism within the mouse locus D12Nyu2.
Identification of a simple sequence polymorphism within the mouse locus D12Nyu2.
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小鼠基因座 D12Nyu2 内简单序列多态性的鉴定。
DOI：
10.1007/bf00360558
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
D'Eustachio,P
中科院分区：
文献类型：
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作者：
Guay-Woodford,LM;Bruns,GA;D'Eustachio,P
最近，完全由简单序列多态性（SSLP）或微卫星组成的小鼠遗传图谱已经构建（Dietrich et al. 1992, 1993）。鉴于其高度多态性，这些标记已成为小鼠遗传图谱的首选工具。然而，SSLP 图谱几乎不包含先前通过限制性片段长度多态性 (RFLP) 绘制的基因或匿名 DNA 片段的标记。因此，在任何给定的感兴趣的杂交中，Southern印迹分析和PCR测定都需要对RFLP和SSLP标记进行类型和精确排序。作为我们定位克隆小鼠先天性多囊肾（cpk）基因座的努力的一部分，我们已经鉴定了与D12Nyu2相关的SSLP，D12Nyu2是一种不与cpk重组的匿名近端染色体（Chr）12标记（Davisson等人199t）。定义 D12Nyu2、M13~)19-25 的探针是由用 AluI 和 RsaI 部分消化的小鼠基因组 DNA 构建的（D'Eustachio 1984）。我们的序列数据表明 M13019-25 中的小鼠基因组 DNA 插入片段发生了广泛的重排。然而，在 1.5 kb 插入片段中，我们鉴定了完整的 332 bp 内部 HindIII 片段（GenBank 登录号 406926）。 Southern 印迹证实该限制性片段映射到小鼠 Chr 12，并对应于小鼠基因组内独特的连续序列（数据未显示）。
Recently, a mouse genetic map consisting entirely of simple sequence polymorphisms (SSLPs) or microsatellites has been constructed (Dietrich et al. 1992, 1993). Given their high degree of polymorphism, these markers have become the tools of choice for genetic mapping in the mouse. However, the SSLP map contains few markers for genes or anonymous DNA fragments previously mapped by restriction fragment length polymorphisms (RFLP). Therefore, in any given cross of interest, both Southern blot analyses and PCR assays are required to type and precisely order RFLP and SSLP markers with respect to each other.As part of our effort to positionally clone the mouse congenital polycystic kidney (cpk) locus, we have identified an SSLP associated with D12Nyu2, an anonymous proximal Chromosome (Chr) 12 marker that does not recombine with cpk (Davisson et al. 199t). The probe that defines D12Nyu2, M13~) 19-25, was constructed from mouse genomic DNA partially digested with AIuI and RsaI (D'Eustachio 1984). Our sequence data imply that the mouse genomic DNA insert in M13019-25 is extensively rearranged. Yet within the 1.5 kb insert, we have identified an intact 332-bp internal HindIII fragment (GenBank accession number 406926). Southern blotting confirmed that this restriction fragment maps to mouse Chr 12 and corresponds to a unique, contiguous sequence within the mouse genome (data not shown).