Molecular basis for the auxin-independent phenotype of crown gall tumor tissues.
Molecular basis for the auxin-independent phenotype of crown gall tumor tissues.
复制标题
冠瘿肿瘤组织生长素独立表型的分子基础。
DOI：
10.1126/science.3511528
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Thomashow,LS
中科院分区：
文献类型：
--
作者：
Thomashow,MF;Hugly,S;Buchholz,WG;Thomashow,LS
将特定的 Ti（肿瘤诱导）质粒序列（T-DNA）从根癌农杆菌转移至多种植物会导致冠瘿瘤的形成。这些组织与大多数植物细胞的不同之处在于它们可以在不添加植物激素的情况下在体外生长。在这里，提供的数据为冠瘿组织的生长素独立表型提供了解释。结果表明，从带有 pTiA6 基因 1 的三种细菌制备的粗无细胞提取物可以将 L-色氨酸转化为吲哚-3-乙酰胺；缺乏基因1的对照提取物不能进行该反应。其他报告表明 pTiA6 基因 2 产物可以将吲哚-3-乙酰胺转化为吲哚-3-乙酸，这是一种天然存在的植物生长素。结论是，冠瘿组织的不依赖生长素的表型涉及引入编码生长素合成的两步途径的Ti质粒序列。
The transfer of specific Ti (tumor-inducing) plasmid sequences, the T-DNA, fromAgrobacterium tumefaciensto a wide range of plants results in the formation of crown gall tumors. These tissues differ from most plant cells in that they can be grown in vitro in the absence of added phytohormones. Here, data are presented that offer an explanation for the auxin-independent phenotype of crown gall tissues. It is shown that crude cell-free extracts prepared from three bacterial species harboring pTiA6 gene 1 could convert L-tryptophan to indole-3-acetamide; control extracts lacking gene 1 could not carry out the reaction. Other reports indicate that the pTiA6 gene 2 product can convert indole-3-acetamide to indole-3-acetic acid, a naturally occurring auxin of plants. It is concluded that the auxin-independent phenotype of crown gall tissue involves the introduction of Ti plasmid sequences encoding a two-step pathway for auxin synthesis.