A and B forms of the androgen receptor are expressed in a variety of human tissues
A and B forms of the androgen receptor are expressed in a variety of human tissues
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DOI：
10.1016/0303-7207(96)03819-1
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发表时间：
1996-06-18
影响因子：
4.1
通讯作者：
McPhaul, MJ
中科院分区：
文献类型：
--
作者：
Wilson, CM;McPhaul, MJ
人生殖器皮肤成纤维细胞含有全长110 K雄激素受体蛋白（AR-B，表观M（r）约为110000）和87 K N-末端截短的AR同种型（AR-A，表观M（r）约为87000）。这两种AR物质在结构上类似于孕酮受体（PR）的A-和B-亚型。我们通过Western印迹分析研究了人类AR亚型在各种胎儿和成人组织中的分布模式。高盐组织提取物中免疫反应性AR蛋白的相对水平通过光密度测定法与标准正常生殖器皮肤成纤维细胞制剂进行比较来估计。高AR水平（AR-A + AR-B = 0.8-7.7）存在于来自中期妊娠胎儿的男性和女性生殖组织中，包括阴茎、前列腺、睾丸、附睾、阴囊皮肤、阴唇皮肤、子宫/宫颈和卵巢。在14种非生殖器官胎儿组织（膀胱、脂肪、肺、大血管、气管、肌肉、头皮皮肤、肾脏、甲状腺、肠、胸腺、输尿管、胃和直肠）中也检测到AR-A和AR-B（0.08-0.9）。AR-A占这些组织中检测到的AR蛋白的4-26%。其他10个胎儿组织的AR-B水平较低（0.02-0.3），AR-A水平很低或没有。AR-B也是在17种成人组织中发现的主要或唯一免疫反应性AR种类。成人生殖组织（前列腺、子宫内膜、卵巢、子宫、输卵管、睾丸：精囊、子宫肌层和射精管）中的AR水平范围为0.1至2.2。在前列腺癌、子宫内膜癌、甲状腺癌和肾癌中也有AR（0.4-0.8）表达。在成人乳腺、结肠、肺和肾上腺标本中检测到较低水平的AR（0.03-0.1）。这项研究表明，免疫反应性AR蛋白存在于各种各样的人胎儿和成人组织中，两种AR亚型在许多组织中表达。
Human genital skin fibroblasts contain both the full-length 110 K androgen receptor protein (AR-B, apparent M(r) approximate to 110000) and an 87 K N-terminally truncated AR isoform (AR-A, apparent M(r) approximate to 87000). These two AR species are structurally analogous to the A- and B-isoforms of the progesterone receptor (PR). We examined the distribution pattern of human AR isoforms in a variety of fetal and adult tissues by Western blot analysis. Relative levels of immunoreactive AR proteins in high salt tissue extracts were estimated by densitometry in comparison to a standard normal genital skin fibroblast preparation. High AR levels (AR-A + AR-B = 0.8-7.7) were present in male and female reproductive tissues from mid-trimester fetuses, including penis, prostate, testis, epididymis, scrotal skin, labial skin, uterus/cervix, and ovary. AR-A and AR-B (0.08-0.9) also were found in 14 non-genital fetal tissues (bladder, fat, lung, great vessel, trachea, muscle, scalp skin, kidney, thyroid, intestine, thymus, ureter, stomach and rectum). AR-A accounted for 4-26% of the AR protein detected in these tissues. Ten other fetal tissues had low levels of AR-B (0.02-0.3) and little or no delectable AR-A. AR-B also was the predominant or only immunoreactive AR species found in 17 adult human tissues. AR levels in adult reproductive tissues (prostate, endometrium, ovary, uterus, fallopian tube, testis: seminal vesicle, myometrium, and ejaculatory duct) ranged from 0.1 to 2.2. Immunoreactive AR (0.4-0.8) also was present in specimens of prostate carcinoma, endometrial carcinoma, thyroid carcinoma and kidney. Lower levels of AR (0.03-0.1) were detected in adult breast, colon, lung and adrenal gland specimens. This study demonstrates that immunoreactive AR protein is present in a wide variety of human fetal and adult tissues and that two AR isoforms are expressed in many tissues.