Regulation of spermidine/spermine N1-acetyltransferase in L6 cells by polyamines and related compounds.
Regulation of spermidine/spermine N1-acetyltransferase in L6 cells by polyamines and related compounds.
复制标题
多胺和相关化合物对 L6 细胞中亚精胺/精胺 N1-乙酰转移酶的调节。
DOI：
10.1042/bj2380581
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Pegg,AE
中科院分区：
文献类型：
--
作者：
Erwin,BG;Pegg,AE
暴露于外源性多胺培养的大鼠L 6细胞导致亚精胺/精胺N1-乙酰转移酶的活性非常大的增加。精胺比亚精胺更有效地带来了这种增加，但在这两种情况下，乙酰转移酶活性的升高增加了亚精胺转化为腐胺的细胞转化。N1-乙酰基转移酶在L 6细胞中的转化非常迅速，亚精胺后的半衰期为9分钟，精胺后的半衰期为18分钟。各种各样的合成多胺类似物也带来了大量的诱导亚精胺/精胺N1-乙酰转移酶活性。这些化合物包括对称-去甲精脒、对称-去甲精胺、对称-高次精脒、N4-取代的精脒衍生物、1，3，6-三氨基己烷、1，4，7-三氨基庚烷和脱氧精胍灵，它们的效力与精脒相当，以及N1 N8-双（乙基）精脒、N1 N9-双（乙基）高次精脒、甲基乙二醛双（乙基）（脒基腙）、乙基乙二醛双（脒基腙）和1，1 ′-[（甲基乙二亚基）二次氮基]双（3-氨基胍）的活性甚至比亚精胺更强。这表明，这些多胺类似物可能带来的细胞多胺的减少，不仅通过抑制生物合成，但通过刺激亚精胺降解成腐胺。
Exposure of rat L6 cells in culture to exogenous polyamines led to a very large increase in the activity of spermidine/spermine N1-acetyltransferase. Spermine was more potent than spermidine in bringing about this increase, but in both cases the elevated acetyltransferase activity increased the cellular conversion of spermidine into putrescine. The N1-acetyltransferase turned over very rapidly in the L6 cells, with a half-life of 9 min after spermidine and 18 min after spermine. A wide variety of synthetic polyamine analogues also brought about a substantial induction of spermidine/spermine N1-acetyltransferase activity. These included sym-norspermidine, sym-norspermine, sym-homospermidine, N4-substituted spermidine derivatives, 1,3,6-triaminohexane, 1,4,7-triaminoheptane and deoxyspergualin, which were comparable with spermidine in their potency, and N1N8-bis(ethyl)spermidine, N1N9-bis(ethyl)homospermidine, methylglyoxal bis(guanylhydrazone), ethylglyoxal bis(guanylhydrazone) and 1,1′-[(methylethanediylidene)dinitrilo]bis(3-amino-guanidine), which were even more active than spermidine. It is suggested that these polyamine analogues may bring about a decrease in cellular polyamines not only by inhibiting biosynthesis but by stimulating the degradation of spermidine into putrescine.