Improved Survival of Full-Thickness Skin Graft With Low-Molecular Weight Heparin-Protamine Micro/Nanoparticles Including Platelet-Rich Plasma
Improved Survival of Full-Thickness Skin Graft With Low-Molecular Weight Heparin-Protamine Micro/Nanoparticles Including Platelet-Rich Plasma
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DOI：
10.1097/sap.0000000000001051
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发表时间：
2017-05-01
影响因子：
1.5
通讯作者：
Kiyosawa, Tomoharu
中科院分区：
文献类型：
--
作者：
Takabayashi, Yuki;Ishihara, Masayuki;Kiyosawa, Tomoharu
背景资料：活化的富血小板血浆分泌许多生长因子（GF）和低分子量肝素-鱼精蛋白微/纳米颗粒LMWH-P M/NPs（LMWH-P M/NPs）与分泌的GFs相互作用，增强并稳定GFs的分泌。目的：研究LMWH-P M/NPs与GFs的作用（来自富血小板血浆）在全层皮肤移植物（FTSG）上的作用。将共96只近交系雄性大鼠麻醉，并在大鼠背部皮肤上造成4 cm（2）全层皮肤伤口。然后将LMWH-PM/NP和GF、LMWH-PM/NP、GF和盐水（对照）均匀地注射到伤口处的皮肤肌肉中。第二天，大鼠接受FTSG。在FTSG后的指定日期，用二维激光多普勒血流计检测FTSG部位（创面床和FTSG）的血流。第10天，拍摄FTSG部位的照片，并评估FTSG存活率。结果：LMWH-PM/NPs和GFs治疗全层皮肤创面，与GFs、LMWH-PM/NPs和对照组相比，能有效提高FTSG的存活率和FTSG部位的血流量。结论：创面预先注射LMWH-PM/NPs和GFs可提高FTSG的存活率，促进FTSG部位的血流和血管生成。
Background: Activated platelet-rich plasma secrets many growth factors (GFs), and low-molecular weight heparin-protamine micro/nanoparticles (LMWH-P M/NPs) significantly interact with, enhance, and stabilize the secreted GFs.Objective: The purpose of this study was to evaluate the effects of LMWH-P M/NPs and GFs (from platelet-rich plasma) on full-thickness skin graft (FTSG).Methods: A total of 96 inbred male rats were anesthetized and 4-cm(2) full-thickness skin wound were created on dorsal skin of rats. LMWH-P M/NPs and GFs, LMWH-P M/NPs, GFs and saline (control) were then injected evenly into cutaneous muscles at the wound. The next day, the rats underwent FTSG. On the indicated days after FTSG, blood flow of FTSG site (wound bed and FTSG) was examined by 2-dimensional laser Doppler blood flowmeter. On 10 days, pictures of FTSG site were taken and FTSG survival rate was evaluated. Histologic analyses of skin samples were performed on 4, 7, and 10 days.Results: Treatment of full-thickness skin wound with LMWH-P M/NPs and GFs effectively promoted survival rate of FTSG and blood flow of FTSG site compared with those treated with GFs, LMWH-P M/NPs, and control. LMWH-P M/NPs and GFs also promoted new vessel formation at FTSG site.Conclusions: The prior injection of LMWH-P M/NPs and GFs into wound bed increases FTSG survival rate, and promotes blood flow and angiogenesis at FTSG site.