Quantitative photoluminescence of broad band absorbing melanins: a procedure to correct for inner filter and re-absorption effects
Quantitative photoluminescence of broad band absorbing melanins: a procedure to correct for inner filter and re-absorption effects
复制标题
DOI：
10.1016/j.saa.2004.08.014
复制
发表时间：
2005-07-01
影响因子：
4.4
通讯作者：
Meredith, P
中科院分区：
文献类型：
--
作者：
Riesz, J;Gilmore, J;Meredith, P
我们报告的光致发光发射和激发光谱的高吸收样品的再吸收和内过滤器的影响进行校正的方法。我们推导出一般形式的校正，并探讨各种方法来确定参数。此外，使用高吸收荧光素和黑色素（宽带吸收）溶液测试校正方法;在应用校正后，恢复了吸收和发射之间的预期线性关系，表明方法有效。这些程序允许在吸收显著的浓度下对低量子产率样品（如黑色素）的发射进行准确的定量分析。(c)2004 Elsevier B. V.保留所有权利。
We report methods for correcting the photoluminescence emission and excitation spectra of highly absorbing samples for re-absorption and inner filter effects. We derive the general form of the correction, and investigate various methods for determining the parameters. Additionally, the correction methods are tested with highly absorbing fluorescein and melanin (broadband absorption) solutions; the expected linear relationships between absorption and emission are recovered upon application of the correction, indicating that the methods are valid. These procedures allow accurate quantitative analysis of the emission of low quantum yield samples (such as melanin) at concentrations where absorption is significant. (c) 2004 Elsevier B.V. All rights reserved.