INTERCARPELLARY GROWTH OF POLLEN TUBES IN THE EXTRAGYNOECIAL COMPITUM AND ITS CONTRIBUTION TO FRUIT SET IN AN APOCARPOUS SPECIES, SCHISANDRA SPHENANTHERA (SCHISANDRACEAE)
INTERCARPELLARY GROWTH OF POLLEN TUBES IN THE EXTRAGYNOECIAL COMPITUM AND ITS CONTRIBUTION TO FRUIT SET IN AN APOCARPOUS SPECIES, SCHISANDRA SPHENANTHERA (SCHISANDRACEAE)
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华中五味子（五味子科）无壳物种雌核外花粉管的心皮生长及其对坐果的贡献
DOI：
10.3732/ajb.1100165
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发表时间：
2012-05-01
影响因子：
3
通讯作者：
Wang, Xiao-Fan
中科院分区：
文献类型：
--
作者：
Du, Wei;Wang, Xiao-Fan
研究结论：离生心皮植物的心皮在雌蕊群中分离。雌蕊群外复合体在基部被子植物中普遍存在，在离心皮植物中，它通过花粉管在心皮间的生长而具有增加后代数量的潜力。到目前为止，在任何物种中，都没有研究过雌蕊群外的复合体对果实或结实的影响。本研究探讨了南五味子相邻心皮之间的花粉管通道及其对座果的影响。方法：收集了数百株盛花期的雌蕊，进行田间坐果率调查。结果表明：花粉粒萌发后，花粉管沿着假花柱表面伸展，花粉管通道的长度和长度随假花柱长度的增加而增加。其中一些转向，并进入胚珠在柱头冠的末端，而其他人随后通过位于未融合的心皮基部的海角连接到相邻的心皮。没有发现试管在容器表面生长。单心皮人工授粉，可使24个以上心皮受精。自然条件下授粉的心皮率显著低于坐果率。结论：同一心皮的花粉管可以在相邻心皮间的雌蕊外密合室中自由杂交。sphenanthera，这种花粉管的心皮间生长可以显着增加离心皮物种的坐果率，至少在S。蝶形花
Premise of the study: Apocarpous plants possess carpels that are separated in the gynoecium. Extragynoecial compita, commonly occurring in basal angiosperms, have been proposed to have the potential to increase offspring quantity in apocarpous species through the intercarpellary growth of pollen tubes. To date, the impact of an extragynoecial compitum on fruit or seed set has not been studied in any species. This study investigated the pollen tube pathway between adjacent carpels and its contribution to fruit set in Schisandra sphenanthera.Methods: We investigated the fruit set ratio in the field and collected hundreds of gynoecia at their full flowering stage. Pollinated carpel ratio and pollen tube pathway observations were performed using fluorescence optics.Key results: Pollen grains germinated and tubes extended along the pseudostyle surface. Some of them turned and entered the ovules at the end of the stigmatic crest, whereas others subsequently grew into neighboring carpels through promontory connections located at the base of the unfused carpels. No tubes were found growing on the surface of the receptacle. More than 24 carpels could be fertilized by pollen tubes from one carpel through hand pollination. The pollinated carpel ratio was significantly lower than the fruit set ratio under natural conditions.Conclusions: Pollen tubes from one carpel can easily cross in the extragynoecial compitum between the adjacent carpels of S. sphenanthera, and this intercarpellary growth of pollen tubes can significantly increase the fruit set of apocarpous species, at least in S. sphenanthera.